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MEDOX-BIO Blotting Systems

MEDOX-BIO Mini Western Blotting System
MEDOX-BIO Mini Dual Western Blotting System
MEDOX-BIO Midi Western Blotting System
MEDOX-BIO Midi Dual Western Blotting System
Accessories
medox MEDOX-BIO Mini Semi Dry Blotting System (10X10cm gel)
medox MEDOX-BIO Midi Semi Dry Blotting System (20X20cm gel)
medox MEDOX-BIO Mini Semi Dry Blotting System Advanced (10X10)
medox MEDOX-BIO Midi Semi Dry Blotting System Advanced (20X20)
medox MEDOX-BIO Southern Blotting System
   
 
Medox-Bio Blotting Systems

Immunoblot commonly referred to as "Western blot" (WB), is used to detect antibodies to specific epitopes of electrophoretically separated subspecies of antigens. Western Blotting or immunoblotting is to determine the relative amounts of the protein present in different samples, with a specific primary antibody. Development of electrophorteic transfer to a suitable membrane, by Towbin and others (Western Blot) now permits the rapid, efficient and quantitative transfer of protein and nucleic acids to immobilizing media.

In these assays, antigens are first electrophoresed and then blotted onto a piece of filter paper. Thus the filter paper is the solid phase to which the antigen is bound. The filter is

blocked and then reacted with appropriate tagged antibodies and then substrate. Spots can then be detected by immuno blotting. A color reaction indicates a positive test. These spots indicate the position of the protein of interest.

The transfer of electrophoretically resolved DNA, RNA, and protein to an immobilizing paper or membrane matrix has become a standard procedure for the sensitive and specific detection of biologically interesting macromolecules. Exact and reproducible replicas of separations are captured on the paper or membrane sheets. After transfer, antigens, antibodies, hormones, receptor proteins, and glycoproteins can be detected by staining or by specific fluorescent (FIA), enzyme-substrate (EIA), or autoradiographic (RIA) assay.

Western blots allow investigators to determine the molecular weight of the protein and to measure the relative amounts of protein present in different samples.

Western Blotting systems are useful for the transfer of protein samples from SDS-PAGE gel to nitrocellulose membrane support with gel cassette at high current. The cassette is submerged in a buffer tank in between anode and cathode cells.

 

 

MEDOX-BIO Mini Western Blotting System

Dimensions: 16cm(L) x 5cm(B) x 13cm(H)
No. Of Cassettes: 1 (8 x 7cm gel)
Buffer Volume: 300ml

Complete unit consists of

  • Basic unit with platinum electrode
  • Gel cassette (1 Set)
  • Filter pad (Pack of 6)
  • Cushion pad (Pack of 6)
  • Power cord and Lid (1 no. each)
  • User manual

Ordering Information

Cat.No

Product

Units

MX-1246-01

MEDOX-BIO Mini Western Blotting System

Set

 

 

MEDOX-BIO Mini Dual Western Blotting System

Dimensions: 16cm(L) x 12cm(B) x 13cm(H)
No. Of Cassettes: 2 (8 x 7cm gel)
Buffer Volume: 700ml

Complete unit consists of

  • Basic unit with platinum electrode
  • Gel cassette (2 Sets)
  • Filtet pad (Pack of 12)
  • Cushion pad (Pack of 12)
  • Heat exchanger (1 No.)
  • Power cord and Lid (1 No. each)
  • User manual
 

Ordering Information

Cat.No

Product

Units

MX-1247-01

MEDOX-BIO Mini Dual Western Blotting System

Set

 

 

MEDOX-BIO Midi Western Blotting System

Dimensions: 18cm(L) x 12cm(B) x 26cm(H)
No. Of Cassettes: 1 (16 x 14cm gel)
Buffer Volume: 1000ml

Complete unit consists of

  • Basic unit with platinum electrode
  • Gel cassette (1 Set)
  • Filter pad (Pack of 6)
  • Cushion pad (Pack of 6)
  • Power cord and Lid (1 No. each)
  • User manual
 

Ordering Information

Cat.No

Product

Units

MX-1248-01

MEDOX-BIO Midi Western Blotting System

Set

 

 

MEDOX-BIO Midi Dual Western Blotting System

Dimensions: 18cm(L) x 12cm(B) x 13cm(H)
No. Of Cassettes: 2 (8 x 7cm gel)
Buffer Volume: 700ml

Complete unit consists of

  • Basic unit with platinum electrode
  • Gel cassette (2 Sets)
  • Filtet pad (Pack of 12)
  • Cushion pad (Pack of 12)
  • Heat exchanger (1 No.)
  • Power cord and Lid (1 No. each)
  • User manual
 

Ordering Information

Cat.No

Product

Units

MX-1247-01

MEDOX-BIO Mini Dual Western Blotting System

Set

 

 

Accessories

Cat. No.

Product

Unit

MEDOX-BIO WESTERN BLOTTING SYSTEMS

MX-1246-02

Scotch Brite Pads 8 x 7cm gel

6/pack

MX-1246-03

Cushion Pads 8 x 7cm gel

12/pack

MX-1246-04

Scotch Pads 16 x 14cm gel

6/pack

MX-1246-05

Cushion Pads 16 x 14cm gel

12/pack

 

 

MEDOX-BIO Mini Semi Dry Blotting System (10X10cm gel)

Dimensions
17cm(L)x17cm(B)x9cm(H)
Buffer Volume: 50ml

Complete unit consists of

  • Basic unit with Graphite Electrode
    assembly (2 Nos.)
  • Insulation Sheets (2 Nos.)
  • Power cord (1 No.)
  • User Manual

Ordering Information

Cat.No

Product

Units

MX-1249-01

MEDOX-BIO® Mini Semi Dry Blotting System (10X10cm gel)

Set

 

 

MEDOX-BIO Midi Semi Dry Blotting System (20X20cm gel)

Dimensions
27cm(L)x27cm(B)x9cm(H)
Buffer Volume: 150ml

Complete unit consists of

  • Basic unit with Graphite Electrode
    assembly (2 Nos.)
  • Insulation Sheets (2 Nos.)
  • Power cord (1 No.)
  • User Manual

Ordering Information

Cat.No

Product

Units

MX-1295-01

MEDOX-BIO® Midi Semi Dry Blotting System (20X20cm gel)

Set

 

 

MEDOX-BIO Mini Semi Dry Blotting System Advanced (10X10)

Ordering Information

Cat.No

Product

Units

MX-1295-01

MEDOX-BIO Mini Semi Dry Blotting System Advanced (10X10)

Set

 

 

MEDOX-BIO Midi Semi Dry Blotting System Advanced (20X20)

Ordering Information

Cat.No

Product

Units

MX-1295-02

MEDOX-BIO Mini Semi Dry Blotting System Advanced (20X20)

Set

 

 

MEDOX-BIO Southern Blotting System

Dimensions
20cm(L)x10cm(B)x7cm(H)
Buffer Volume: 300ml

Complete unit consists of
Basic unit (1 No.)
Weight (1 No.)
User Manual

Ordering Information

Cat.No

Product

Units

MX-1254-01

MEDOX-BIO® Southern Blotting System (20X20cm gel)

Set


Accessories

Cat.No

Product

Units

MX-1246-02

Filter Pads 8x7cm gel

6/pack

MX-1246-03 Cushion Pads 8x7cm gel 12/pack
MX-1246-04 Filter Pads 16x14cm gel 6/pack
MX-1246-05 Cushion Pads 16x14cm gel 12/pack
 

 

 
   
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