Home| Register/Login |Sitemap
¤    Recognition accorded to our in-house R&D unit by DSIR (Dept of Scientific and Industrial Research), Ministry of Science & Technology, Govt. of India    ¤    Academic Projects for students (Final Year), Periodical Training programme for students are commenced     ¤    Introducing fully moulded 'ELECTRO-POUR' Gel Running Systems.    ¤    Our MEDOX-CATALOGUE 2012-13 is ready for distribution. Please order your copy today    ¤    ISO 9001 : 2008 Certified    ¤
 
 
rayanz.com - Free web site statistics
Personal homepage website counter
  
   Buffers, Staining and Fixing Solutions

Tris (Tris (Hydroxymethyl) Aminomethane)

Tris Buffer pH 8.8
Tris Buffer pH 6.8
Bis-Tris
Sodium Dodecyl Sulfate(SDS)
10% SDS
Tris-Tricine SDS buffer 10X

Tris-Borate EDTA buffer 5X

Western Blotting buffer 5X

Sample buffer for SDS-PAGE 5X
Sample buffer for Native PAGE 5X
Brilliant Blue G stain 2X
Brilliant Blue R stain 2X
Amido Black stain 2X
Ponceau's stain 2X
Destainer 4X

 
Tris (Tris (Hydroxymethyl) Aminomethane)

Biotechnology Grade
MW 121.14
Purity: >99.8%
Moisture: <1.0%
Insolubles: <0.005%
Solubility (10%, water): Clear and haze-free
A280 (1.0M, water): <0.05
As: <0.0005% Pb: <0.0001%
Fe: <0.0001% Cu: <0.0001%
Mg: <0.0001%
DNase, RNase, Protease: None detected

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0941-01

Tris-Base

100g

MX-0941-02

Tris-Base

500g

MX-0941-03

Tris-Base

1kg

MX-0941-06

Tris-Base (Biotech Grade)

1kg

 

 

Tris Buffer pH 8.8

Tris HCl 1.5M pH8.8 is a buffer useful for casting separating gel.

Storage: 4 ° C

Ordering Information

Cat.No

Product

Units

MX-0942-01

Tris Buffer pH8.8

100ml

 

 

Tris Buffer pH 6.8

Tris HCl 1.0M pH6.8 is a buffer useful for casting stacking gel.

Storage: 4 ° C

Ordering Information

Cat.No

Product

Units

MX-0943-01

Tris Buffer pH6.8

100ml

 

 

Bis-Tris

Bis-(2-Hydroxymethyl)imino-tris(hydroxymethyl) Methane
Ultra Pure Grade
MW 209.2 Purity: >99.0%
pKa ( 25 ° C): 6.50±0.2
Melting range: 104 ± 2 ° C

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0224-01

Bis-Tris

50g

MX-0224-02

Bis-Tris

100g

MX-0224-03

Bis-Tris

250g

 

 

Sodium Dodecyl Sulfate(SDS)

Biotechnology Grade
MW 288.38
Purity: >99.0%
A260 (3%,water): <0.1 A280 (3%,water): <0.1
Chloride: <0.1% Phosphate: <0.005%
Pb: <0.005% Cu: <0.005%
DNase, RNase: Not detected

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0944-01

Sodium Dodecyl Sulphate (SDS)

100g

MX-0944-02

Sodium Dodecyl Sulphate (SDS)

500g

 

 

10% SDS

Sodium Dodecyl Sulphate 10g/100ml, the ionic detergent which binds strongly to the proteins.

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0945-01

10% SDS

50 ml

 

 

Tris-Glycine SDS buffer 10X concentrate

This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.025M Tris, 0.192M glycine and 0.1%SDSpH 8.3-8.6.

Storage: 4 ° C

Ordering Information

Cat.No

Product

Units

MX-0946-01

Tris-Glycine SDS Buffer 10X Concentrate

1litre

 

 

Tris-Glycine buffer Native PAGE 10X

This buffer is used as tank buffer for Native PAGE. The buffer at 1X concentration contains 0.025M Tris and 0.192M Glycine pH 8.3-8.6.

Storage: 4 ° C

Ordering Information

Cat.No

Product

Units

MX-0947-01

Tris-Glycine Buffer Native PAGE 10X

1litre

 

 

Tris-Tricine SDS buffer 10X

This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.1M Tris, 0.1M Tricine and 0.1% SDS pH 8.3-8.6.

Storage: 4 ° C

Ordering Information

Cat.No

Product

Units

MX-0948-01

Tris-Tricine SDS Buffer 10X

250 ml

 

 

Tris-Borate EDTA buffer 5X

This buffer is used as Agarose Gel Electrophoresis buffer. The buffer at 1X concentration contains 0.02MTris, 0.1M Borate and 1mM EDTA pH7.6

Storage: 4 ° C

Ordering Information

Cat.No

Product

Units

MX-0949-01

Tris-Borate EDTA Buffer 5X

200 ml

 

 

Western Blotting buffer 5X

This buffer is used as electro-transfer buffer for protein blotting from SDS-PAGE. The buffer at 1x concentration contains 15mM Tris and 120mM Glycine, 0.1% SDS and 20% Methanol pH 8.6.

Storage: 4 ° C

Ordering Information

Cat.No

Product

Units

MX-0950-01

Western Blotting Buffer 5X

500ml

 

 

Sample buffer for SDS-PAGE 5X

Sample buffer at 5x concentration contains 4% SDS, 20% Glycerol, 10% b -mercaptoethanol, 0.004% bromophenol blue and 0.125M Tris-HCl, pH approximately 6.8. This is used for preparing sample for SDS-PAGE.

Storage: -20 ° C

Ordering Information

Cat.No

Product

Units

MX-0951-01

Sample Buffer for SDS PAGE 5X

5 x 12ml

 

 

Sample buffer for Native PAGE 5X

Sample buffer at 5X concentration contains 20% Glycerol, 0.004% bromophenol blue and 0.125M Tris-HCl pH approximately 6.8. This is used for preparing sample for Native PAGE.

Storage: -20 ° C

Ordering Information

Cat.No

Product

Units

MX-0952-01

Sample Buffer for Native PAGE 5X

5 x 12 ml

 

 

Brilliant Blue G stain 2X

Content of one bottle of brilliant blue G stain is to be made to a final volume of 1litre with deionized H 2 O. The final solution will contain 0.1% (w/v) brilliant blue G, 25% methanol and7%Acetic acid. This is used for staining protein in PAGE and Agarose gels.

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0953-01

Brilliant Blue G Stain 2X

500 ml

 

 

Brilliant Blue R stain 2X

Content of one bottle of brilliant blue R stain is to be diluted to one litre with deionized H 2 O. The final solution contains 0.25% (w/v) brilliant blue R, 40% methanol and 7% Acetic acid. This is suitable for staining protein in polyacrylamide and agarose gels.

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0954-01

Brilliant Blue R Stain 2X

500 ml

 

 

Amido Black stain 2X

After dilution to 1X concentration the solution will contain 0.1% (w/v) amido black, 25% (w/v) isopropanol and 10% Acetic acid. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE visualizes proteins in

microgram range with a clear background.

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0955-01

Amido Black Stain 2X

250 ml

 

 

Ponceau's stain 2X

After dilution to 1X concentration the solution will contain 0.2% ponceau's, 3%TCA. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE. Can visualize proteins in microgram range with a clear background.

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0956-01

Ponceau's Stain 2X

250 ml

 

 

Destainer 4X

Content of one bottle of destainer is to be diluted 4 times with deionized water. The final solution contains 15% methanol and 7.5% Acetic acid. This is suitable for destaining polyacrylamide and agarose gels.

Storage: Room Temperature

Ordering Information

Cat.No

Product

Units

MX-0957-01

Destainer 4X

1 litre

 

 

 
 
© 2006 Medox Biotech India Pvt. Ltd. All Rights Reserved. |