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MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Bacteria)
MEDOX-Easy Bacterial Genomic DNA Extraction Kit
(Quick One Step Method)
MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Blood)
MEDOX-Easy Whole Blood DNA Extraction Kit (Plant)
MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit
(Animal & Mammalian Tissue)
MEDOX-Easy Genomic Plant DNA Extraction Kit
MEDOX-Easy Genomic Plant DNA Extraction Kit (CTAB Method)
MEDOX-Easy Plant DNA Extraction for PCR Amplification
 
 
MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Bacteria)
(For isolation of genomic DNA from bacterial cells)
 

Principle
This kit is designed for fast isolation of genomic DNA from bacteria. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.

Applications

  • Genomic DNA purification from different sources
  • PCR
  • Southern Blotting
  • RAPD, AFLP or RFLP

Features

  • Preparation of high quality genomic DNA from different sources.
  • Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
  • High yield and Reproducible
  • No phenol / chloroform extraction , no ethanol precipitation

Procedure
Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.

0.8% Agarose Gel. Genomic DNA preparations from bacterial samples using the Kit. The left lane is DNA Marker.


Kit Components

MX-0611-01
(50 Preps)

MX-0611-02
(100 Preps)

Digestion Solution

20ml

40ml

Wash Solution

12ml

24ml

Elution Buffer

5ml

10ml

Proteinase K

2mg

4mg

MX-10 Column

50

100

2.0ml Collection Tube

50

100

Protocol

1

1


Ordering Information

Cat.No

Product

Units

MX-0611-01

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Bacteria)

20 preps

MX-0611-02

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Bacteria)

50 preps

 

MEDOX-Easy Bacterial Genomic DNA Extraction Kit
(Quick One Step Method )
 

Principle
This kit is designed as a quick one step method for DNA extraction from bacteria. This isolated DNA act as DNA template for PCR amplification. This yield enough to 50 PCR reactions set up.

Features & Application

  • The rapid isolation of DNA from both gram-positive and gram-negative organisms.
  • The isolated DNA is suitable for medical diagnosis of PCR amplifications.
  • No binding resin-avoids shearing
  • No RNase treatment required
  • Does not involve organic solvent extractions. (ex. Phenol, chloroform)

Kit Components & Storage

DNA Extraction solution
Kit Protocol

Ordering Information

Cat.No

Product

Units

MX-0657-01

MEDOX-Easy Bacterial Genomic DNA Extraction Kit (Quick One Step Method)

100 extractions

 

 

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit, Blood
(For automated isolation of genomic DNA from blood samples)
 

Principle
This MX-10 spin column mini-preps kit is designed for a fast isolation of genomic DNA from clinical samples such as fresh or frozen whole blood (with common anti-coagulants such as citrate, EDTA, and heparin), serum, bone, other body fluids and cell suspensions. 200µl-400µl per column of healthy whole blood sample is enough to obtain 6-12µg of pure genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the MX-10 Column. Purified blood genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.

Applications

  • Extraction of Genomic DNA from whole blood and other body fluids.

Features

  • High quality of genomic DNA from blood and other clinical samples
  • High yield and Reproducible
  • No phenol / chloroform extraction , no ethanol precipitation

Storage
With the exception of the Proteinase K, the kit may be stored at room temperature. The proteinase K should be stored at -20ºC. The kit is stable for 12 months at room temperature. For longer storage, keep all contents cold.

0.8%Agarose Gel. GenomicDNApreparations from a single blood samples of whole human blood using the Kit. The left lane is DNA Marker.


Kit Components

MX-0607-01
(50 Preps)

MX-0607-02
(100 Preps)

TBP Buffer

120ml

2 x 120ml

TBM Buffer

25ml

50ml

TE (pH 8.0)

15ml

30ml

Proteinase K

2mg

4mg

Wash Solution

12ml

24ml

Elution Buffer

5ml

10ml

MX-10 Column

50

100

2.0ml Collection Tube

50

100

Protocol

1

1


Ordering Information

Cat.No

Product

Units

MX-0607-01

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Blood)

50 preps

MX-0607-02

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Blood)

100 preps

 

 

MEDOX-Easy Whole Blood DNA Extraction Kit
 

Principle
This kit is used for rapid isolation and effectively reproducible, high yield og genomic DNA from blood sample.

Features & Application

  • The DNA extracted from 200 ul of blood sample.
  • The isolated DNA is highly pure form, its suitable for PCR applications.
  • No binding resin-avoids shearing
  • No RNase treatment required
  • Does not involve organic solvent extractions. (ex. Phenol, chloroform)

Kit Components & Storage

Cell lysis buffer
DNA Extraction solution
Kit Protocol

Ordering Information

Cat.No

Product

Units

MX-0658-01

MEDOX-Easy Whole Blood DNA Extraction Kit

50 extractions

 

 

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit
(Animal & Mammalian Tissue)
(For isolation of high-molecular-weight DNA from a wide range of samplers)
 

Principle
This kit is designed for fast isolation of genomic DNA from animal tissues. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides, proteins, salts and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.

Applications

  • Genomic DNA purification from different sources
  • PCR
  • Southern Blotting
  • RAPD, AFLP or RFLP

Features

  • Preparation of high quality genomic DNA from variable sources.
  • Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
  • High yield and Reproducible
  • No phenol / chloroform extraction , no ethanol precipitation


Procedure

Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.

Gel electrophoresis of a animal genomic DNA sample purified by using EZ-10 Spin Column Genomic DNA Minipreps Kit. The lane at the left end is DNA marker.


Kit Components

MX-0608-01
(20 Preps)

MX-0608-02
(50 Preps)

ACL Solution

20ml

40ml

AB Solution

20ml

40ml

PBS Solution

20ml

40ml

Proteinase K

20mg

40mg

Wash Solution

12ml

24ml

Elution Buffer

5ml

10ml

MX-10 Column

50

100

2.0ml Collection Tube

50

100

Protocol

1

1


Ordering Information

Cat.No

Product

Units

MX-0608-01

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Animal & Mammalian Tissue)

10 preps

MX-0608-02

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Animal & Mammalian Tissue)

20 preps

MX-0608-03

MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Animal & Mammalian Tissue)

50 preps

 

 

MEDOX-Easy Genomic Plant DNA Extraction Kit
(For isolation of DNA from plant samples)
 

Principle
This kit is designed for fast isolation of genomic DNA from plant tissues. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides,proteins, salts and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.

Applications

  • Genomic DNA purification from different sources
  • PCR
  • Southern Blotting
  • RAPD, AFLP or RFLP

Features

  • Preparation of high quality genomic DNA from variable sources.
  • Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
  • High yield and Reproducible
  • No phenol / chloroform extraction, no ethanol precipitation


Procedure

Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.

Gel electrophoresis of a plant genomic DNA sample purified by using MX-10 Spin Column Genomic DNA Minipreps Kit. The lane at the left end is DNA marker.


Kit Components

MX-0623
(50 Preps)

MX-0624
(100 Preps)

PCL Solution

15ml

30ml

PP Solution

2ml

4ml

PB Solution

20ml

40ml

Wash Solution

12ml

24ml

Elution Buffer

5ml

10ml

MX-10 Column

50

100

2.0ml Collection Tube

50

100

Protocol

1

1


Ordering Information

Cat.No

Product

Units

MX-0612-01

MEDOX-Easy Genomic Plant DNA Extraction Kit

10 preps

MX-0612-02

MEDOX-Easy Genomic Plant DNA Extraction Kit

50 preps

 

 

MEDOX-Easy Genomic Plant DNA Extraction Kit (CTAB Method)
 

The Plant material is suspended in CTAB genomic DNA extraction buffer. Cells are then lysed by osmotic shock and salt precipitate the cell debris. Proteins are then precipitated using phenol chloroform and followed by ethanol precipitation of DNA.

Features & Application

  • Mainly used to prepare pure high molecular weight DNA for gene manipulation.
  • Used to construct genomic DNA libraries and to probe the plant genome for the presence of DNA markers such as RAPD and RFLP.

Ordering Information

Cat.No

Product

Units

MX-0612-03

MEDOX-Easy Genomic Plant DNA Extraction Kit (CTAB Method)

10 preps

MX-0612-04

MEDOX-Easy Genomic Plant DNA Extraction Kit (CTAB Method)

50 preps

 

 

MEDOX-Easy Plant DNA Extraction for PCR Amplification
 

Principle
This kit is designed as a quick one step method for DNA extraction from bacteria. This isolated DNA act as DNA template for PCR amplification. This yield enough to 50 PCR reactions set up.

Features & Application

•  Rapid isolation of DNA from monocot and dicot leaves.
•  The highly pure isolated DNA is suitable for PCR application.
•  No binding resin - avoids shearing
•  No RNase treatment required
•  Does not involve organic solvent extractions (ex. Phenol, chloroform)

Kit Components & Storage

Cell lysis buffer
DNA Extraction solution
Kit Protocol

Ordering Information

Cat.No

Product

Units

MX-0612-05

MEDOX-Easy Plant DNA Extraction for PCR Amplification

100 extractions

 

 

   
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