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MEDOX-BIOTM Transformation Teaching Kit

MEDOX-BIOTM Competent Cell Preparation Teaching Kit
MEDOX-BIOTM Bacterial Conjugation Teaching Kit
MEDOX-BIOTM Transduction Teaching Kit
MEDOX-BIOTM Phage Titration Teaching Kit
MEDOX-BIOTM Transposon-Transposition Teaching Kit
MEDOXBIO MEDOX-BIO® Isolation And Characterization Of Petite Mutants Of Yeast Teaching Kit
MEDOXBIO MEDOX-BIO® AMES Test Teaching Kit
MEDOXBIO MEDOX-BIO® Study Of Physical And Chemical Mutagens On The Survival Of E.coli Growth Teaching Kit
MEDOXBIO MEDOX-BIO® Isolation of Antibiotic Resistant and Auxotrophic Mutant Teaching Kit
 
MEDOX-BIOTM Transformation Teaching Kit

This method involves the transformation of a plasmid vector into a host and studying the expression. The Plasmid contains a gene that codes for b-galactosidase enzyme (lac-z). The expresssion of this gene is induced by IPTG. This enzyme converts the chromogenic substrate in medium (X-Gal) forming an intense blue colour.

Features & Application
* Easy method of competent cell preparation and to screen the recombinant.
* Enables students to learn about a-complimentation.

Kit Components & Storage
(Enough to perform 5/20 Experiments)

LB Broth Medium (RT) Agar (RT)
CaCl2 (4°C) CaCl2 + Glycerol (4°C)
Ampicillin (4°C) X-Gal (-20°C)
IPTG (-20°C) Plasmid DNA (-20°C)
Bacterial Host (4°C) Kit Protocol



Ordering Information

Cat.No

Product

Units

MX-1170-01

MEDOX-BIOTM Transformation Teaching Kit
(by Blue-White Selection Method)

5 Exps.

MX-1170-02

MEDOX-BIOTM Transformation Teaching Kit
(by Blue-White Selection Method)

20 Exps.

 

 

MEDOX-BIOTM Competent Cell Preparation Teaching Kit

Since DNA is a very hydrophilic molecule, it won't normally pass through a bacterial cell's membrane. In order to make bacteria take in the plasmid, they must first be made "competent" to take up DNA. This is done by creating small holes in the bacterial cells by suspending them in a solution with a high concentration of calcium. DNA can then be forced into the cells by incubating the cells and the DNA together on ice, placing them briefly at 42°C (heat shock), and then putting them back on ice. This causes the bacteria to take in the DNA. The cells are then plated out on antibiotic containing media.

Features & Application
* To maintain lab stock of highly efficient competent cells for plasmid/cosmid transformations.
* More useful in molecular sequencing, Transformation techniques, and Recombination technology.

Kit Components & Storage
(Enough to perform 10 / 20 Experiments)

Ampicillin (4°C) Bacterial Host (4°C)
Solution-I (4°C) Solution-II (4°C)
LB broth powder (RT) Agar powder (RT)
Kit Protocol  

Ordering Information

Cat.No

Product

Units

MX-1172-01

MEDOX-BIOTM Competent Cell Preparation Teaching Kit

10 Exps.

MX-1172-02

MEDOX-BIOTM Competent Cell Preparation Teaching Kit

20 Exps.

 

 

MEDOX-BIOTM Bacterial Conjugation Teaching Kit

Conjugation is one of the methods of gene transfer between two bacterial strains of donor and recipient bacterial cell through direct contacts. By using antibiotic markers the transferred recombinant genes can be identified.  

Features & Application

* Screening of conjugants is based on Antibiotic resistants or susceptibility.

Kit Components & Storage
(Enough to perform 5/20 Experiments)

Antibiotic (4°C) Agar (RT)
Donor Strain A (4°C) LB Broth (RT)
Recipient Strain B (4°C) Kit Protocol



Ordering Information

Cat.No

Product

Units

MX-1145-01

MEDOX-BIOTM Bacterial Conjugation Teaching Kit

5 Exps.

MX-1145-02

MEDOX-BIOTM Bacterial Conjugation Teaching Kit

20 Exps.

 

 

MEDOX-BIOTM Transduction Teaching Kit

Transduction is a process by which bacterial DNA is moved from one bacterium to another by a Virus. When Bacteriophages (Viruses that infect a bacteria) infect a bacterial cell, their normal mode of reproduction is to harness the DNA replication meelinary of the host bacteria cell and make numerous copies of their DNA (or) RNA.
This results in a permanent alteration of the recipient cell genotype.

Features & Application
This kit gives hands on practice to students about transduction with easy procedure.

Kit Components & Storage
Enough to perform 5 / 20 Experiments)

E.coli strain I (donor) (4°C)  
E.coli strain II (recipient) (4°C)  
Phage virulant (4°C) LB broth (RT)
MC buffer (RT) Citrate buffer (RT)
10X salt buffer (RT) Antibiotics (4°C)

Ordering Information

Cat.No

Product

Units

MX-1143-01

MEDOX-BIOTM Transduction Teaching kit

5 Exps.

MX-1143-02

MEDOX-BIOTM Transduction Teaching kit

20 Exps.

 

 

MEDOX-BIOTM Phage Titration Teaching Kit

Phage titration enables the ennumeration of Phage particles on basis of plaque formation on a susceptible host grown. Serial dilution of phage suspension is carried out in 9 tubes.
The phage dilution are then mixed with E.coli and plated onto a hard agar and then visualized for PFU (Plaque Forming Unit).

Features & Application
* To teach students about the plaque forming property of the phage on a specific host.

Kit Components & Storage
(Enough to perform 5 / 20 Experiments)

Bacterial Host (4°C) Phage Lysate (4°C)
1.5ml Microfuge tubes (RT) Agar (RT)
Tryptone (RT) Potassium Chloride (RT)
Sodium Chloride (RT) Calcium Chloride (RT)
Nutrient Broth Medium (RT) Kit Protocol

Ordering Information

Cat.No

Product

Units

MX-1142-01

MEDOX-BIOTM Phage Titration Teaching Kit

5 Exps.

MX-1142-02

MEDOX-BIOTM Phage Titration Teaching Kit

20 Exps.

 

 

MEDOX-BIOTM Transposon-Transposition Teaching Kit

Tn5 transposon is a 5,818bp composite transposon consisting of two inverted repeats of 1533bp (IS 50L and IS 50R) flanking a unique region which carries 3 genes that confers host resistance to the antibiotics kanamycin (Npt II-neomycinphospho transferaseII), Bleomycin and streptomycin. These genes are organized in an operon and are transcribed from a promoter located at the inside end of IS50L .IS 50R encode the transposase responsible for Tn5 transposition. Random mutagenesis involves the introduction of Tn5 into bacterial species of interest via transformation, transduction or conjugation using 'suicide' plasmid or phage vectors, followed by selection for antibiotic resistance markers carried by Tn5.

Features & Application


* To demonstrate Tn5 insertion mutation in the genome of bacteria. Transposition
* In vivo gene manipulation and gene cloning.
* Detection of mutation and gene insertion.

Kit Components & Storage
(Enough to perform 5 / 20 Experiments)

Sup+E.Coli (4°C) l : Tn5 lysate (4°C)
SM Buffer(4°C) Kanamycin (4°C)
l Sensitive supO E.coli culture(4C) LB agar (RT)



Ordering Information

Cat.No

Product

Units

MX-1186-01

MEDOX-BIOTM Transposon-Transposition Teaching Kit

5 Exps.

MX-1186-02

MEDOX-BIOTM Transposon-Transposition Teaching Kit

20 Exps.

 

 

MEDOX-BIOTM Mutation Deduction and Analysis(MUTAGENESIS)Teaching Kit

A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations. Mutation may occur due to detection, insertion or duplication of bases. Mutation can be detected by restriction enzyme mapping. Insertion, deletion or duplication of a base may
create a new restriction enzyme site or change the old one. When such a mutants DNA is digested with a particular restriction enzyme a different restriction map is generated and compared to that of a non-mutated sample. This technique is used in this kit.

Ordering Information

Cat.No

Product

Units

MX-1171-01

MEDOX-BIOTM Mutation Deduction And Analysis
(Mutagenesis) Teaching Kit

5 Exps.

MX-1171-01

MEDOX-BIOTM Mutation Deduction And Analysis
(Mutagenesis) Teaching Kit

10 Exps.

MX-1171-01

MEDOX-BIOTM Mutation Deduction And Analysis
(Mutagenesis) Teaching Kit with Submarine Gel Electrophoresis and Power Supply

5 Exps.

 

 

MEDOX-BIOTM Transformation Of Yeast Cell Teaching Kit
(Using Lithium Acetate)

The selection of plasmids in yeast is based on the use of auxotropic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine). Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Features & Application
* To isolate the transformed colonies (selection of plasmids) from yeast cells.
* This method is very much useful in recombination and gene cloning.

Kit Components & Storage
(Enough to perform 5 / 20 Experiments)

Transformation buffer (RT) PLATE buffer (RT)
Yeast culture (ura-) (4°C) Kit Protocol
Yeast synthetic Drop-out medium (RT)  
Control yeast plasmid DNA pRS316 (ura+) (-20°C)  


Ordering Information

Cat.No

Product

Units

MX-1194-01

MEDOX-BIOTM Transformation of Yeast Cell Teaching Kit
(Using Lithium Acetate)

20 Exps.

 

 

MEDOX-BIO® Isolation And Characterization Of Petite Mutants Of Yeast Teaching Kit

Ordering Information

Cat.No

Product

Units

MX-1544-01

MEDOX-BIO® Isolation And Characterization Of Petite Mutants Of Yeast Teaching Kit

10 Exps.


 

 

MEDOX-BIO® AMES Test Teaching Kit

Ordering Information

Cat.No

Product

Units

MX-1523-01

MEDOX-BIO® AMES Test Teaching Kit

5 Exps.

 

 

MEDOX-BIO® Study Of Physical And Chemical Mutagens On The Survival Of E.coli Growth Teaching Kit

Ordering Information

Cat.No

Product

Units

MX-1524-01

MEDOX-BIO® Study Of Physical And Chemical Mutagens On The Survival Of E.coli Growth Teaching Kit

5 Exps.



 

 

MEDOX-BIO® Isolation of Antibiotic Resistant and Auxotrophic Mutant Teaching Kit

Ordering Information

Cat.No

Product

Units

MX-1072-01

MEDOX-BIO® Isolation of Antibiotic Resistant and Auxotrophic Mutant Teaching Kit

5 Exps.

 

 

 
   
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