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MEDOX-Easy Cleanup Minipreps Kit
MEDOX-Easy Spin Column DNA Gel Extraction Kit
Fast Ligation kit (10 Minutes Ligation)
A-Tailing kit
Single Step Ultra-Competent Cell Preps Kits
MEDOXBIO MEDOX-BIO® PCR Amplication of b-Galactosidase Gene Teaching Kit
MEDOXBIO MEDOX-BIO® AFLP Teaching Kit
   
 
 
First Strand cDNA Synthesis Kit I (AMV)
(For the first strand cDNA synthesis from mRNA template )
 

Description
Using the 1st Strand cDNA Synthesis Kit, RNA is reversibly transcribed into single-stranded cDNA (1). In this method, AMV Reverse Transcriptase synthesizes the new cDNA strand at a site(s) determined by the type of primer used: at the 3'-end of the poly(A)-mRNA when Oligo-p(dT) is used as a primer, at nonspecific spots along the RNA template when using the Random Primer p(dN) 6 , or at a primer-binding site for a sequence-specific primer. The resulting first strand cDNA can then be used as a template for PCR.

AMV Reverse Transcriptase is well suited for the preparation of cDNA for use as a PCR template. Fully active in PCR buffer, AMV Reverse Transcriptase features a high specific activity, ensuring large amounts of template. At 42ºC, this highly processive enzyme is more efficient than M-MuLV Reverse Transcriptase.

Quality Control
The product is tested functionally using 12.5pg of total HOX RNA as the template for amplification of a 674-bp segment of b -actin mRNA (40 cycles). A minimum of 25ng of the RT-PCR product was obtained under these conditions.

Storage and Stability
All components are stable for at least one year when stored at 20 ° C. Avoid repeated freeze/thaw cycles for RNase Inhibitor and Reverse Transcriptase.

Kit Components

MX-0628-01
(10 reactions)

MX-0628-02
(20 reactions)

5X Reaction Buffer
50µl
100µl
dNTP Mix,10mM each
30µl
60µl
Oligo-p(dT)0.5µg/µl
15µl
30µl
Random Primer p(dN)6, 0.2µg/µ
15µl
30µl
RNase Inhibitor, 20U/µl
15µl
30µl
AMV Reverse Transcriptase
200U
400U
RNase-freeddH2O
1.5ml
1.5ml
Protocol
1
1

 

Ordering Information

Cat.No

Product

Units

MX-0628-01

First Strand cDNA Synthesis Kit I (AMV)

10 rxns

MX-0628-02 First Strand cDNA Synthesis Kit I (AMV) 20 rxns
 

 

 
First Strand cDNA Synthesis Kit II (M-MuLV)
(For the first strand cDNA Synthesis from mRNA template)
 

Description
Kit is designed for preparation of full-length first strand cDNA from RNA templates. The first strand cDNA synthesis kit relies on a cloned enzyme, Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV RT). M-MuLV RT synthesizes first strand cDNA at sites determined by the type of primer used:

  • Random hexamer primer: at non-specific points along an RNA template. In this case, all RNA in a population are templates for cDNA synthesis.
  • Oligo(dT) 18 : at the 3*-end of poly(A) + mRNA. In this case, only mRNA with 3*-poly(A) tails are templates for cDNA synthesis.
  • Sequence specific primer: at a primer-binding site.

First strand cDNA synthesized with this system can be used as a template in the polymerase chain reaction (PCR). As the reaction conditions of cDNA synthesis and PCR are compatible, cDNA reaction mixture can be added directly to a PCR mixture. The kit is optimized for maximum yield of full length cDNA. The addition of ribonuclease inhibitor lowers the risk of mRNA degradation during the reaction.

The first strand cDNA synthesized may also be used as a template for second strand synthesis. Radiolabeled DNA can be used as a probe in hybridization experiments.

As most users do not need to do control First cDNA synthesis, this kit do not include control RNA.

KitComponents MX-0620-01
(10 reactions)
MX-0620-02
(20 reactions)

5X Reaction Buffer

50µl
100µl
dNTP Mix,10mM each
30µl
60µl
Oligo-(dT)18 0.5µg/µl
15µl
30µl
Random Primer p(dN)6, 0.2µg/µl
15µl
30µl
RNase Inhibitor, 20U/µl
15µl
30µl
M-MuLV Reverse Transcriptase, 20U/µl
200U
400U
RNase-freeddH2O
1.5ml
1.5ml
Protocol
1
1

Notes

  • Multiple freezing/thawing of RNA should be avoided. Thaw and keep control RNA on ice.
  • It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% diethylpyrocarbonate (DEPC) (soak overnight in 0.1% aqueous solution of DEPC at 37 ° C, then heat at 100 ° C for 30min and autoclave).
  • Wearing gloves is highly recommended.
  • One unit of M-MuLV RT incorporates 1nmole of deoxyribonucleotide into DE81 absorbable form in 10min at 37°C.
  • One unit of ribonuclease inhibitor is defined as the amount of protein required to inhibit 50% of the activity of 5ng of RNase A.

Ordering Information

Cat.No

Product

Units

MX-0620-01

First Strand cDNA Synthesis Kit II (M-MuLV)

10 rxns

MX-0620-02 First Strand cDNA Synthesis Kit II (M-MuLV) 20 rxns

 

 
MEDOX-Easy Spin Column PCR Products Purification Kit ( For purification of PCR products and DNA cleanup of variable enzymatic reactions.)
 

Principle
This kit utilizes a technology which adsorbs selectively up to 10µg PCR DNA or DNA fragments in the presence of specialized binding buffers. Primers (<40-mer), nucleotides, enzymes, mineral oil and other impurities do not bind to the column. PCR products or DNA fragments can be eluted with elution buffer in small volume. (1) If PCR mixture contains non-specific amplified DNA fragments, PCR product should be purified by agarose gel. In this case, DNA Gel Extraction Kit is recommended. (2) This kit is not apable of removing the template DNA or primers with length longer than 40-mer.

Applications

  • Recovery of PCR products from PCR reaction mixture.
  • Recovery of DNA fragments from enzymatic reaction solutions.
  • DNA clean up & removal of nucleotides and dye terminator.

Features

  • Rapid and economical. Entire procedure takes less than 20 minutes.
  • High yields (60-80%). It is suitable to recover 100 bp-40 kb DNA fragments.
  • Efficient removal of contaminants. Purified DNA can be used in any downstream applications such as sequencing, labeling, restriction enzymatic digestions, ligations or transformations.
  • No phenol / chloroform extraction or ethanol precipitation.

Quality Control
All components of the kit are tested in recovery of 10µg of Lambda DNA/ Hind III + Eco R I from the solution.

Procedure
The kit uses a simple bind-wash-elute procedure. PCR products or enzymatic reactions are mixed with the appropriate binding buffer and pass through to the column. DNA binds to the silica-based membrane. Impurities such as enzymes, salts, nucleotides and

primers (< 40-mer) are washed away. Pure DNA is eluted in a small volume of the low-salt elution buffer or water. Ready for use in any downstream applications.

Diagram

Gel electrophoresis of the PCR products from a single sample purified by using MX-10 Spin Column. The right lane is DNA marker.



KitComponents
MX-0601-01
(50 Preps)
MX-0601-02
(100 Preps)

Binding Solution I

20ml

40ml

Wash Solution
12ml
24ml
Elution Buffer
5ml
10ml
EZ-10 Column
50
100
2.0ml Collection Tube
50
100
Kit Protocol
1
1

 

Ordering Information

Cat.No

Product

Units

MX-0601-01

MEDOX-Easy Spin Column PCR Products Purification Kit

50 preps

MX-0601-02 MEDOX-Easy Spin Column PCR Products Purification Kit 100 preps
MX-0616-01 MEDOX-Easy 96-well Spin Column PCR Products Purification Kit 5 x 96 well plate
MX-0616-02 MEDOX-Easy 96-well Spin Column PCR Products Purification Kit 10x 96 well plate

 

 

 
MEDOX-Easy Cleanup Minipreps Kit (For a fast cleanup of upto 10µg of DNA fragments from variable enzymatic reactions)
 

Principle
This kit provides a simple, efficient method for purification of DNA fragments from variable enzymatic reactions such as cDNA synthesis, ligation, restriction digestions, tailing, PCR, alkaline phosphatase, nick translation, dye terminators products from PCR reaction mixture. It is also an ideal tool to desalt the solution of DNA as well as to remove residual organic solvents or unincorporated or primers (<40-mer) from reaction mixture. This utilizes a technology which adsorbs selectively up to 10µg DNA fragments in the column in the presence of specialized binding buffers. Primers (<40-mer), nucleotides, enzymes, mineral oil and other impurities do not bind to the column. DNA fragments can be eluted readily with elution buffer in small volume.

Applications

  • DNA cleanup from the enzymatic reactions.
  • Removal of nucleotides and primers (<40-mer).

Features

  • Rapid and economical. Entire procedure takes less than 20 minutes.
  • High yield (60-80%). It is suitable to recover 100 bp-40 kb DNA fragments.
  • Efficient removal of contaminants. Purified DNA can be used in any downstream applications such as sequencing, labeling, restriction enzymatic digestions, ligations or transformations.
  • No phenol / chloroform extraction or ethanol precipitation.

Quality Control
All components of the kit are tested in purification of PCR products from PCR mixture.

Procedure
The kit uses a simple bind-wash-elute procedure. PCR products or enzymatic reactions are mixed with the appropriate binding buffer and pass through the column. DNA binds to the silica-based membrane. Impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA is eluted in a small volume of the low-salt elution buffer or water. Ready for use in any downstream applications.

Gel electrophoresis of the PCR products from a single sample purified by using the Kit. The left lane is DNA marker.

KitComponents
MX-0603-01
(50 Preps)
MX-0603-02
(100 Preps)

Cleanup Solution

20ml

40ml

Wash Solution
12ml
24ml
Elution Buffer
5ml
10ml
MX-10 Column
50
100
Elution Buffer
5ml
10ml
Protocol
1
1

 

Ordering Information

Cat.No

Product

Units

MX-0603-01

MEDOX-Easy Cleanup Minipreps Kit

50 preps

MX-0603-02

MEDOX-Easy Cleanup Minipreps Kit

100 preps

MX-0613-01

MEDOX-Easy 96-well Cleanup Minipreps Kit

5 x 96 well plate

MX-0613-02

MEDOX-Easy 96-well Cleanup Minipreps Kit

10x 96 well plate

 

 

 
MEDOX-Easy Spin Column DNA Gel Extraction Kit
(For extraction and purification of DNA fragments from agarose gels.)
 

Principle

The kit provides a simple and efficient method for DNA extraction from agarose gel. It is also an ideal tool to desalt the solution of DNA as well as to remove residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixture. The kit utilizes a technology which adsorbs selectively up to 10µg DNA fragments in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the column. DNA fragments can be eluted readily with elution buffer.

Application

  • Recovery of DNA fragments from agarose gels.
  • Removal of nucleotides and primers (<40-mer)
  • Recovery of PCR products from reaction solution

Features

  • Rapid and Economic. Entire procedure takes about 20 minutes.
  • High yield (60-80%). It is suitable to recover 100bp-40kb DNA fragments.
  • Efficient removal of contaminants. Purified DNA can be used in any downstream applications such as sequencing, labeling, restriction enzymatic digestions, ligations, hybridization, or transformations.
  • No phenol / chloroform extraction or ethanol precipitation

Quality Control

All components of the kit are tested in purification of 1kb DNA from agarose gel and directly from PCR mixture.

Procedure
The kit uses a simple solubilization-bind-wash-elute procedure. Solubilized agarose gel slices or enzymatic reactions are mixed with the appropriate binding buffer and pass through the column. DNA binds to the silica-based membrane. Impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA is eluted in a small volume of the low-salt elution buffer or water, ready for use in any downstream applications.

DNA fragments with different sizes extracted from a TAE Agarose gel with MX-10 Spin Column Gel Extraction Kit. The lane at right end is DNA fragments before extraction.

Recovery yields are of approximately 60-80%.

Kit Components
MX-0609
(50 Reactions)
MX-0610
(100 Reactions)

MX-10 Column

50
100
Fast DNA ligase 2.0ml Collection tube
50
100
Binding Solution II
30ml
60ml
Wash Solution
12ml
24ml
Elution Buffer
5ml
10ml
Protocol
1
1

 

Ordering Information

Cat.No

Product

Units

MX-0605-01

MEDOX-Easy Spin Column DNA Gel Etraction Kit

50 preps

MX-0605-02 MEDOX-Easy Spin Column DNA Gel Etraction Kit 100 preps

 

 

 

Fast Ligation kit (10 Minutes Ligation)
 

Features

  • Fast 10 minutes for both cohesive and blunt ends.
  • Economic

Application

  • Suitable for cohesive or blunt ends and all common ligation reactions.

Procedure
1. To a 50ng of vector and insert (3 fold excess of insert is recommended) is added DD-water or TE buffer to make final volume as 10µl

2. Add 10µl of 2X Fast Ligation Buffer and mix .

3. Add 1µl of Fast Ligase and Mix thoroughly. Centrifuge briefly. The mixture is kept at room temperature for 5 minutes. Keep on ice for transformation or store at -20 ° C

Note : Inactivation by heating is not recommended, may reduce transformation efficiency. Avoid repeated freeze/thaw cycles.

Quality Control
The Fast Ligation Kit is tested for transformation efficiency by a comparison with regular ligation procedure. Only 10% of difference is observed.

Kit Components
MX-0639
(25 Reactions)
MX-0640
(100 Reactions)

2X reaction buffer

250µl
1ml

Fast DNA ligase
25µl
100µl
Protocol
1
1

 

Ordering Information

Cat.No

Product

Units

MX-0621-01

Fast Ligation Kit

25 rxns

MX-0621-02 Fast Ligation Kit 100 rxns

 

 

 

A-Tailing Kit (For adding a single A to blunt-ended of PCR fragments or any other double-stranded DNA fragments.)
 

Principle
To clone blunt-ended PCR fragments generated by Pfu DNA polymerase or other enzymes to T-Vector, A-tailing reaction is required. This kit provides Toogle DNA polymerase and dATP mixture for adding single A to these blunt-ended DNA fragments. A tailed DNA fragments can easily be prepared within 10 minutes.

Features

  • Rapid and Economical

Application
Add single A to blunt-ended of PCR fragments or any other double-stranded DNA fragments

Procedure
To 100ng of blunt-ended DNA fragment is added 5µl of 10X A-Tailing Buffer, 1µl DATP and 5units of Taq DNA polymerase. The mixture is incubated at 72 ° C for 5-10minutes.

Above DNA fragment with additional A residue at 3' end can be cleaned up by using Spin Column PCR products Purification Kit. (MX-0601).

Kit Components
MX-0622-01
(40 Reactions)
MX-0622-02
(100 Reactions)

Toogle DNA polymerase

250 units
600 units
10X A-tailing buffer
0.5ml
2 x 0.5ml
dATP 10mM
50µl
120µl
Protocol
1
1

 

Ordering Information

Cat.No

Product

Units

MX-0622-01

A-Tailing Kit

40 rxns

MX-0622-02 A-Tailing Kit 100 rxns

 

 

 

 
Single Step Ultra-Competent Cell Preps Kit
 

Description
SSCS Solution is supplied as a ready to use solution
Transformation and storage solution ( SSCS ), enables researchers to prepare competent E.coli cells in a single step. SSCS has been reported to be faster and easier than other methods of producing competent cells, such as the traditional CaCl 2 method described by Sambrook et al. , or other high competency protocols. Transformation efficiencies depend on the strain of E.coli , as well as the nature and quality of the transformed DNA. A typical transformation efficiencies is 10 7 ~10 8 transformants/µg DNA. For example, the transformation efficiencies observed for E.coli strains DH1, DH5 a , Hb101, JM109, LE392, MM294, SCS-1, XL1-blue and TG1 ranged from 1.5 x 10 7 to 1.0 x 10 8 per µg of DNA.

Applications

  • •  Preparation of E.coli competent cells for transformation.

Application

  • Simple & Fast Entire procedure takes about 5 minutes.
  • Stable at -70 ° C with little or no loss in transformation efficiency. It is suitable for long term storage
  • No heat shock.
  • High transformation efficiencies of 10 6 -10 8 transformants/µg DNA are typically obtained.
  • Reproducible

Ordering Information

Cat.No

Product

Units

MX-0623-01

Single Step Ultra-Competent Cell Preps Kit

25ml

MX-0623-02 Single Step Ultra-Competent Cell Preps Kit 50ml

 

MEDOX-BIO® PCR Amplication of b-Galactosidase Gene Teaching Kit

Ordering Information

Cat.No

Product

Units

MX-1149-01

MEDOX-BIO® PCR Amplication of b-Galactosidase Gene Teaching Kit

5Exps.

 

 

MEDOX-BIO® AFLP Teaching Kit

Ordering Information

Cat.No

Product

Units

MX-1183-01

MEDOX-BIO® AFLP Teaching Kit

5Exps.

 

 

 
   
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