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MEDOX - Easy Ultrapure Spin Column Plasmid DNA Minipreps Kit
(For purification of plasmid DNA from culture)
Principle

This kit provides a simple and efficient method for Mini plasmid DNA purification. DNA fragments are selectively adsorbed in MX-10 column and other impurities such as proteins, salts and nucleotides are washed away. Plasmid DNA can be eluted in a small volume of Tris Buffer. Plasmid DNA purified with this kit can be used for any downstream experiments.

Note: This kit is used for preparation of up to 10µg of pure plasmid DNA. If large scale of preparation is needed, MX-200 column and MX-500 column are recommended.

Applications
  • Plasmid DNA purification.
  • Recovery of DNA fragments from reaction solutions. Removal of nucleotides and oligos (<40 - mer) & plasmid DNA clean up.
Features
  • Simple and fast. The entire procedure takes about 10-15 minutes.
  • Preparation of high quality plasmid DNA from bacterial culture. Purified DNA can be used in any downstream applications such as sequencing, transformation, restriction enzymatic digestion and ligation.
  • High yield (>80%) and reproducible.
  • No phenol / chloroform extraction or ethanol precipitation.
Quality Control

All components in the kit are tested in purification of 10µg pUC 18 DNA from overnight culture.

Procedure

Bacterial cultures are first lysed, the lysates are cleared by routine method. Plasmid DNA from the cleared lysates is selectively bound to the silica based membrane in the MX-10 column and impurities are washed away. Pure plasmid DNA is eluted in low-salt buffer or water.

Kit Components
MX-0606-01
(50 Preps)
MX-0606-02
(100 Preps)

Solution I

6ml

12ml

Solution II*

12ml

24ml

Solution III

25ml

50ml

Wash Solution**

12ml

24ml

Elution Buffer

5ml

10ml

RNase A (10mg/ml)

120µl

240µl

MX-10 Column

50

100

2.0 ml Collection Tube

50

100

Protocol

1

1

* Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 ° C.
** Before use for the first time, add four times of 96%-100% of ethanol to Wash Solution (v/v).

Ordering Information

Cat.No

Product

Units

MX-0606-01

MEDOX-Easy Ultrapure Spin Column Plasmid DNA Minipreps Kit

50 preps

MX-0606-02

MEDOX-Easy Ultrapure Spin Column Plasmid DNA Minipreps Kit

100 preps

MX-0602-02 MEDOX-Easy 96 Well Spin Column Plasmid DNA Minipreps Kit 10x 96 well plate
MX-0618-01 MEDOX-Easy 96-well Classic Plasmid DNA Minipreps Kit 5 x 96 well plate
MX-0618-02 MEDOX-Easy 96-well Classic Plasmid DNA Minipreps Kit 10x 96 well plate
MX-0609-01 MEDOX-Easy Ultrapure Spin Column Plasmid DNA Midipreps Kit 10 preps

 

 

   
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