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Principle
This kit is designed for fast isolation of total RNA from bacteria, yeast, fungi, plant and animal tissues. This kit can also be used to clean up RNA, which are isolated by different methods or after enzymatic reactions. The kit contains a membrane embedded in a spin column for binding upto 10µg of RNA. Nucleotides, proteins, salts and other impurities do not bind to the MX-10 Column.
Applications
- Preparation of Total RNA from variable sources.
- RT-PCR
- Differential display
- cDNAsynthesis
- PolyA + RNA selection
- RNase/S1 nuclease protection
Features
- Preparation of High quality total RNA from animal cells or other sources.
- Rapid and Economical. Entire procedure takes about 20 minutes (Timing for preparation of samples is not included).
- High yield and Reproducible
- No phenol / chloroform extraction , no ethanol precipitation
Procedure
The detailed procedures for Isolation of Total RNA from Animal Cells, Bacteria, Tissue, Plant Cells and Filamentous Fungi and for RNA clean up are supplied with kits.
Storage
The kit is stable for 12 months at room temperature. For longer storage, keep all the kit contents in cold condition.
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Gel electrophoresis of the total RNA from the leaf purified by using Spin Column |
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Gel electrophoresis of the total RNA from the bacteria purified by using Spin Column |
* Solution A may form a precipitate upon storage. If necessary,
re-dissolve it by warming it
at 37 ° CSolution B should be kept at 2-8 ° C after receiving and usage.
Kit Components |
MX-0617-01
(5 Preps) |
MX-0617-02
(20 Preps) |
MX-0617-03
(100 Preps) |
RLT Solution A |
3.5ml |
14ml |
70ml |
RW Solution |
3 ml |
12ml |
60ml |
RPE Solution B |
1.25ml |
5ml |
22ml |
DEPC-Water |
0.25ml |
1ml |
5ml |
EZ-10 Column |
5 |
20 |
100 |
2.0ml Collection Tube |
5 |
20 |
100 |
Protocol |
1 |
1 |
1 |
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Ordering Information |
Cat.No |
Product |
Units |
MX-0617-01 |
MEDOX-Easy Spin Column Total RNA Minipreps Super Kit |
5 preps |
MX-0617-02 |
MEDOX-Easy Spin Column Total RNA Minipreps Super Kit |
20 preps |
MX-0617-03 |
MEDOX-Easy Spin Column Total RNA Minipreps Super Kit |
100 preps |
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