Principle
This kit is designed for fast isolation of total RNA from bacteria, yeast, fungi, plant and animal tissues. This kit can also be used to clean up RNA, which are isolated by different methods or after enzymatic reactions. The kit contains a membrane embedded in a spin column for binding upto 10µg of RNA. Nucleotides, proteins, salts and other impurities do not bind to the MX-10 Column.

Applications

• Preparation of Total RNA from variable sources.
• RT-PCR
• Differential display
• cDNAsynthesis
• PolyA + RNA selection
• RNase/S1 nuclease protection

Features

• Preparation of High quality total RNA from animal cells or other sources.
• Rapid and Economical. Entire procedure takes about 20 minutes (Timing for preparation of samples is not included).
• High yield and Reproducible
• No phenol / chloroform extraction , no ethanol precipitation

Procedure
The detailed procedures for Isolation of Total RNA from Animal Cells, Bacteria, Tissue, Plant Cells and Filamentous Fungi and for RNA clean up are supplied with kits.

Storage
The kit is stable for 12 months at room temperature. For longer storage, keep all the kit contents in cold condition.

	Gel electrophoresis of the total RNA from the leaf purified by using Spin Column
	Gel electrophoresis of the total RNA from the bacteria purified by using Spin Column

* Solution A may form a precipitate upon storage. If necessary, re-dissolve it by warming it
at 37 ° CSolution B should be kept at 2-8 ° C after receiving and usage.

Principle
This kit provides a convenient and stright forward method for purification of mRNA from cells or tissues. The kit contains a guanidine-thiocyanate based buffer for efficient cell and tissue lysis, protein denaturation and RNase inactivation to keep mRNA intact. Homogenized cells or tissue lysates containing total RNA are incubated with Oligo-dT. mRNA is selectively absorbed on the Oligo-dT cellulose and other impurities are washed away. The mixture containing mRNA. Oligo-dT Cellulose is placed on the MX-10 column. While mRNA is released by R-Elution Buffer in small volume. This kit is used for 100 mg of tissue sample or 10 6 cells.

Applications

• RNA blotting
• In vitro translation
• Differential display
• CDNA synthesis or library construction
• RT-PCR
• Microinjection

Features

• Fast and simple
• Reproducible

Storage
The kit is stable for 12 months at room temperature.

Description

• Total RNA Extraction Reagent (TRIZOL) is a ready- to-use reagent for the isolation of total RNA from cells and tissues.

• This technique performs well with small quantities of tissue (50~100mg) and cells (5 x 10 6 ), and large quantities of tissue (>1g) and cells (>10 7 ), of human, animal, plant, or bacterial origin.

• Total RNA Extraction Reagent (TRIZOL) facilitates isolation of a variety of RNA species of large or small molecular size.

Storage Conditions: 2~8°C
Total RNA Extraction Reagent (TRIZOL) has demonstrated stability of 12 months when stored at 2~8°C for optimal performance.

The T-Vector PCR Product Cloning Kit is suitable for cloning of PCR products with additional A at 3' end. The special ligation system provided by the kit enable customs to finish ligation in 1 hour.

PUCm-T vector is designed for simplifying cloning of PCR products. Many thermal stable DNA polymerase, PCR products amplified by DNA polymerase such as Taq , Tth DNA produce additional A at 3' end which could be easily ligated to T vector with additional T.

PUCm-T is a kind of noval pUC derivative T vector, the multiple restriction sites with most of them single site and adjusted -galactose reading frame make it easy for screening target clone through blue and white plaque. Specially designed two Pst 1 sites beside inserted fragments make it easy for screening target clone by Pst 1 digestion, at same time inserted fragments also could be screened by cheap and efficient restriction enzymes such as EcoR 1 and Hind III double digestion. Inserted fragments also could be sequenced using universal primers M13 and T7 promoter primer. In vitro transcription could be processed through site of T7 RNA polymerase promoter in pUCm-T.

Kit Components MX-0650-01 (1 ug) MX-0650-02 (5 ug) PUCm-T vector(50 ng/ul) 1 ug/20ul 5 ug/100ul 10X Ligation Buffer 50 ul 300 ul 50% PEG4000 50 ul 300 ul T4 DNA ligase 5U/ul 100 U 500 U Sterilized ddH2O 1ml 1ml Protocol 1 1

Ordering Information Cat.No Product Units MX-0650-01 MEDOX-Easy Instant Vector Cloning Kit 1ug MX-0650-02 MEDOX-Easy Instant Vector Cloning Kit 5 ug