Genomic DNA from different sources are prepared through Spin columns based methods. These purified high molecular weight Genomic DNA can be used in most molecular biology experiments including Restriction digestion, PCR, Southern blotting etc.

Storage Buffer : 10mM Tris-HCl(pH 8.0) and 1mM EDTA

Storage : -20°C

pUC18 DNA is isolated from E.coli strain DH5 a . Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double-stranded, 2686 base pairs in length.

Concentration : 0.3-0.5mg/ml

Storage Buffer : Tris-HCl (pH7.8) and 1mM EDTA.

Storage : -20°C

pUC19 DNA is isolated from E.coli strain DH5 a . Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double- stranded, 2686 base pairs in length.

Concentration :200µg/ml

Storage Buffer : 10mM Tris-HCl (pH7.8) and 1mM EDTA.

Storage : -20°C

pBR322 DNA is isolated from E.coli (dam+, dcm+) and purified by ultra centrifugation. The molecule is a double-stranded circle, with 4361 base pairs in length.

Concentration :200µg/ml

Storage Buffer : 10mM Tris-HCl(pH7.8) and 1mM EDTA

Storage : -20°C