Electrophoresis Reagents

Products

Nucleic Acid Electrophoresis Reagents

Recommended for Nucleic acid fragments with sizes >1000bp. This agarose is also suitable for blotting assays. DNase / RNase activity not detected.

Storage: Room Temperature

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Cat.No Product Units
MX-0906-01 Agarose Low EEO 50g
MX-0906-02 Agarose Low EEO 100g
MX-0906-03 Agarose Low EEO 500g
MX-0906-04 Agarose Low EEO 1Kg

Recommended for serum protein electrophoresis and immuno electrophoresis.
DNase / RNase activity not detected.

Storage: Room Temperature

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Cat.NoProductUnits
MX-0907-01Agarose Medium EEO50g
MX-0907-02Agarose Medium EEO100g
MX-0907-03Agarose Medium EEO1Kg

Recommended for immuno electrophoresis. DNase / RNase activity not detected.

Storage: Room Temperature

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MX-0908-01Agarose High EEO50g
MX-0908-02Agarose High EEO100g
MX-0908-03Agarose High EEO1Kg

Recommended for 1000bp fragment separation in analytical electrophoresis. The low melting temperature of this agarose (65 ° C) allows the gel to be melted without damaging the DNA, so the fragments can be recovered. Dnase / Rnase activity not detected.

Storage: Room Temperature

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MX-0905-01Agarose Low melting point10g
MX-0905-02Agarose Low melting point100g

Prehybridization buffer is 2X in concentration, the buffer is prepared in a nuclease free water, it contains SSC buffer, sonicated Salmon sperm DNA, Denharts solution.

Application:

In pre-hybridization step, filter (membrane) is incubated in a prehybridization buffer, which is designed to precoat or block the membrane, then probe bind non-specifically to the template DNA. In hybridization pre-hybridization step is important, fail to do this step leads to high background.

Storage: Prehybridization buffer is supplied in frozen condition

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Cat.No Product Units
MX-0653-01 Prehybridization Buffer 2X 50 ml

Hybridization buffer is 2X in concentration, the buffer is prepared in a nuclease free water, it contains SSC buffer, Denharts solution.

Application:

In Hybridization step, filter (membrane) is incubated in a hybridization buffer, with probe.

Storage: Hybridization buffer is supplied in frozen condition.

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MX-0654-01Hybridization Buffer 2X50 ml

A single-strength (1X) solution contains 0.089M Trisbase, 0.089M borate and 0.002M EDTA.

Application:

10X TBE used as running buffer for agarose gel electrophoresis at the working concentration of either 0.5X or 1X

Storage: Room Temperature.

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MX-0900-0110X TBE Buffer200ml

A single strength (1X) solution contains 0.04M Tris base, 0.04M acetate, and 0.001M EDTA/p>

Application:

50X TAE used as running buffer for agarose gel electrophoresis at the working concentration of 1X

Storage: Room Temperature.

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MX-0900-01 10X TBE Buffer 200ml

It consists of 0.025% Bromophenol Blue and 40% Sucrose, used as a tracking dye for agarose gel electrophoresis at the concentration of 1X.

Storage: 4 ° C

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MX-0902-016X Gel Loading Dye6 x 1ml

Biotechnology Grade

MW 394.32

Purity: >98.0%

Moisture: <8.0%

Ammonium Bromide: <0.1%

Storage: Room Temperature

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MX-0904-01Ethidium Bromide (Powder)10mg
MX-0904-02Ethidium Bromide (Powder)50mg
MX-0904-03Ethidium Bromide (Powder)100mg
MX-0904-04Ethidium Bromide (Powder)1g

A readily prepared solution and can be used as such. It acts as an intercalating agent to nucleic acids, which fluorescence under UV (to stain nucleic acid).

Storage: Room Temperature

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MX-0904-03Ethidium Bromide (Solution)1ml

It is used in gel and running buffer to visualize DNA.
The migration of the DNA can be monitored, as bands are visible in ambient light.

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MX-0971-0110X Gel Staining Solution (for Nucleic Acid)100 ml

it consists of 0.025% Orange G and 30% Glycerol. Used as a tracking dye for agarose gel electrophoresis/p>

Storage : 4°C

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Cat.NoProductUnits
MX-0903-016X Orange Gel Loading Dye6X1ml

DNA Markers

100, 200, 300, 400, 500×3, 600, 700, 800, 900 and 1000bp.

The intensity of the 500bp band is increased to yield an internal reference indicator
Concentration : 200µg/ml

Storage Buffer : 1XTE (10mMTris-HCl, pH8.0, 1.0mM EDTA)

6X Loading Buffer : 30% Glycerol,
0.12% bromophenol blue,0.12% xylene cyanol

Storage: -20°C

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Cat.NoProductUnits
MX-0916-01100bp DNA Ladder (10 Fragments)10µg
MX-0916-02100bp DNA Ladder (10 Fragments)50µg
MX-0916-03100bp DNA Ladder (10 Fragments)100µg
MX-0916-04100bp DNA Ladder (10 Fragments)250µg

100, 200, 300, 400, 500×3, 600, 700, 800, 900, 1000 x2 and 1500bp

The intensities of the bands of 500bp, 1000bp are increased to yield internal reference indicators.

Concentration : 200µg/ml

Storage Buffer :1XTE (10mMTris-HCl, pH8.0, 1.0mM EDTA)

6X Loading Buffer : 30% Glycerol,

0.12% bromophenol blue, 0.12% xylene cyanol

Storage: -20°C

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Cat.No Product Units
MX-0916-01 100bp DNA Ladder (11 Fragments) 10µg
MX-0916-02 100bp DNA Ladder (11 Fragments) 50µg
MX-0916-03 100bp DNA Ladder (11 Fragments) 100µg
MX-0916-04 100bp DNA Ladder (11 Fragments) 250µg

9 DNA fragments: 100, 200, 300, 400, 500, 600, 800,1000 and 1500bp.

Concentration : 540ng / 5µl

Recommended loading volume : 5µl

Storage Buffer : 1XTE (10mMTris-HCl, pH8.0, 1.0mM EDTA)

6X Loading Buffer :

30% Glycerol 0.12% bromophenol blue, 0.12% xylene cyanol

Storage: -20°C

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Cat.No Product Units
MX-0917-01 100bp DNA Ladder (Quantitative) 100 run
MX-0917-02 100bp DNA Ladder (Quantitative) 5 x 100 run
MX-0918-01 100bp DNA Ladder (Quantitative) Ready to use 100 run
MX-0918-02 100bp DNA Ladder (Quantitative) Ready to use 5 x 100 run

16 DNA fragments: 100, 200, 300, 400, 500, 600, 800, 1000, 1500, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8kb, and 10 kb

DNA Logical-Ladder is consisted of 16 DNA fragments. The DNA Logic-Ladder was formulated to yield such a pattern that each band contains a Logic amount of the DNA fragment that correlates to its size. The Logic-ladder not only reveals a unique pattern for easy recognition of the bands on the gel, but also gives a quantitative gradient of the DNA amount. Thus, to measure both the size and the quantity of a particular DNA fragment could be achieved at a glance.

Recommended loading volume : 5µl

Storage Buffer : 1XTE (10mM Tris-HCl, pH8.0, 1mM EDTA)

6X Loading Buffer :

30% Glycerol,

0.12% bromophenol blue,

0.12% xylene cyanol

Storage: -20°C

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Cat.No Product Units
MX-0919-01 100bp-10 Kb DNA Logical Ladder 100 run
MX-0920-01 100bp-10 Kb DNA Logical Ladder (Ready to use) 100 run

9 DNA fragments: 0.5kb, 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6kb, 8kb and 10kb.

DNA 1Kb ladder is consisted of the 9 DNA fragments. The intensity of the 3kb band has been increased to yield an internal reference indicator.

Concentration : 250ng / 5µl

Recommended loading volume: 5µl

Storage Buffer : 1XTE (10mM Tris-Hcl, pH8.0, 1mM EDTA)

6x Loading Buffer :

30% Glycerol,

0.12% bromophenol blue,

0.12% xylene cyanol

Storage: -20°C

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Cat.NoProductUnits
MX-0910-011 Kb DNA Ladder (0.5-10Kb DNA Ladder)100 run
MX-0911-011 Kb DNA Ladder (0.5-10Kb DNA Ladder) (Ready to Use)100 run

Six DNAFragments as 3530*,4878, 5643, 5804,7421 and 21,226*bp Supplied with 6X Loading dye solution

Lambda DNA (cI857,Sam7) was completely digested by EcoRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH7.6) and1mM EDTA.

EcoRI digest of Lambda DNA yields the following 6 DNA fragments

Concentration : 0.3-0.5mg/ml

6X Loading Dye Solution :

0.25% bromophenol blue, 0.25% xylene cyanol, 60% glycerol and 60mm EDTA.

Storage: -20°C

*Note: The fragments marked by * may form additional bands, can be separated by heating 65°C for 5 min and then cooling on the ice for 3min.

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Cat.No Product Units
MX-0913-01 Lambda DNA / Eco RI Marker 50µg
MX-0913-02 Lambda DNA / Eco RI Marker 100µg

7 DNA fragments: 23130*, 9416, 6557, 4361*, 2322, 2027 & 564bp

Lambda DNA was completely digested by HindIII, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH7.6) and 1mM EDTA. Hind III digest of Lambda DNA yields 7 DNA fragments

Concentration: 550ng / 6µl

Recommend loading volume: 6µl

Storage Buffer :

10mM Tris-HCl (pH8.0) and 1mM EDTA, 0.02% bromophenol blue, 0.02% xylene cyanol and 5% glycerol

Storage: -20°C

*Note : The cohesive ends (12bp cos site of bacteriophage Lambda) of fragments 23130bp and 4361bp may anneal to and form the additional band. Fragments (marked by *) may be separated by heating to 65°C for 5 min and then cooling on ice for 3 min.

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Cat.No Product Units
MX-0914-01 Lambda DNA / Hind III Marker, Ready to use 100 run

14DNA Fragments: 117 224, 702, 1264, 1371, 1929, 2323, 3675, 4324, 4822, 5687*, 6369, 7242, 8453*bp

0.05mg for 100 applications
Supplied with 6X Loading dye solution

Lambda DNA was completely digested by both EcoR 911, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

*Note: The fragments marker may form additional bands, can be separated by heating to 65°C for 5mins and then cooling on the ice for 3mins.

Concentration : 0.3-0.5mg/ml

Storage Buffer : 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

6X Loading Dye Solution:

0.25% bromophenol blue,

0.25% xylene cyanol,

30% glycerol and 50mM EDTA.

Storage: -20°C

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Cat.NoProductUnits
MX-0924-01Lambda DNA / Eco R911 ( Bst EII) Marker50µg

12 DNA Fragments: 564, 831, 947, 1375, 1584, 1904, 2027, 3530*, 4268, 4973, 5148, 21226bp

0.05mg for 100 applications

Supplied with 6X Loading dye solution Lambda DNA was completely digested by both EcoRI and HindIII , phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

EcoRI & HindIII digest of Lambda DNA yields 12 DNA fragments

Storage Buffer: 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

6X Loading Dye Solution:

0.25% bromophenol blue,

0.25% xylene cyanol,

30% glycerol and 50mM EDTA.

Storage: -20°C

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Cat.No Product Units
MX-0915-01 Lambda DNA / Eco RI+ Hind III Marker 50µg
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Cat.NoProductUnits
MX-1816-01100bp DNA Ladder Prime50 run
MX-1816-02100bp DNA Ladder Prime100 run
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Cat.NoProductUnits
MX-1817-01100bp DNA Ladder Select50 run
MX-1817-02100bp DNA Ladder Select100 run
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Cat.NoProductUnits
MX-1818-01100bp DNA Ladder Mid50 run
MX-1818-02100bp DNA Ladder Mid100 run
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Cat.NoProductUnits
MX-1819-01100bp DNA Ladder Short50 run
MX-1819-02100bp DNA Ladder Short100 run
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MX-1820-011kb DNA Ladder Prime50 run
MX-1820-021kb DNA Ladder Prime100 run
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Cat.NoProductUnits
MX-1821-01500bp DNA Ladder50 run
MX-1821-02500bp DNA Ladder100 run
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Cat.NoProductUnits
MX-1822-01200bp DNA Ladder50 run
MX-1822-02200bp DNA Ladder100 run
MX-1823-01200bp DNA Ladder Select100 run
MX-1823-02200bp DNA Ladder Select100 run
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Cat.NoProductUnits
MX-1824-0150bp DNA Ladder50 run
MX-1824-0250bp DNA Ladder100 run
MX-1825-0150bp DNA Ladder Prime50 run
MX-1826-0250bp DNA Ladder Prime100 run
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Cat.NoProductUnits
MX-1827-0120bp DNA Ladder50 run
MX-1827-0220bp DNA Ladder100 run
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Cat.NoProductUnits
MX-1828-0110bp DNA Ladder50 run
MX-1828-0210bp DNA Ladder100 run
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Cat.NoProductUnits
MX-1830-01DNA Ladder Low Range50 run
MX-1830-02DNA Ladder Low Range100 run
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Cat.NoProductUnits
MX-1831-01DNA Ladder Mid Range50 run
MX-1831-02DNA Ladder Mid Range100 run
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Cat.NoProductUnits
MX-1832-01DNA Ladder High Range (DH1)50 run
MX-1832-02DNA Ladder High Range (DH1)100 run
MX-1833-01DNA Ladder High Range (DH2)50 run
MX-1833-02DNA Ladder High Range (DH2)100 run
MX-1834-01DNA Ladder High Range (DH3)50 run
MX-1834-02DNA Ladder High Range (DH3)100 run

Protein Electrophoresis Reagents

Tris HCl 1.5M pH8.8 is a buffer useful for casting separating gel.

Storage: 4 ° C

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Cat.NoProductUnits
MX-0942-01Tris Buffer pH8.8100ml

Tris HCl 1.0M pH6.8 is a buffer useful for casting stacking gel.

Storage: 4 ° C

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Cat.NoProductUnits
MX-0943-01Tris Buffer pH6.8100ml

Bis-(2-Hydroxymethyl)imino-tris(hydroxymethyl) Methane
Ultra Pure Grade
MW 209.2 Purity: >99.0%
pKa ( 25 ° C): 6.50±0.2
Melting range: 104 ± 2 ° C

Storage: Room Temperature

Ordering Information
Cat.NoProductUnits
MX-0224-01Bis-Tris50g
MX-0224-02Bis-Tris100g
MX-0224-03Bis-Tris250g

Biotechnology Grade
MW 288.38
Purity: >99.0%
A260 (3%,water): <0.1 A280 (3%,water): <0.1
Chloride: <0.1% Phosphate: <0.005%
Pb: <0.005% Cu: <0.005%
DNase, RNase: Not detected

Storage: Room Temperature

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Cat.NoProductUnits
MX-0944-01Sodium Dodecyl Sulphate (SDS)100g
MX-0944-02Sodium Dodecyl Sulphate (SDS)500g

Sodium Dodecyl Sulphate 10g/100ml, the ionic detergent which binds strongly to the proteins.

Storage: Room Temperature

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Cat.NoProductUnits
MX-0945-0110% SDS50 ml

This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.025M Tris, 0.192M glycine and 0.1%SDSpH 8.3-8.6.

Storage: 4 ° C

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Cat.NoProductUnits
MX-0946-01Tris-Glycine SDS Buffer 10X Concentrate1litre

This buffer is used as tank buffer for Native PAGE. The buffer at 1X concentration contains 0.025M Tris and 0.192M Glycine pH 8.3-8.6.

Storage: 4 ° C

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Cat.NoProductUnits
MX-0947-01Tris-Glycine Buffer Native PAGE 10X1litre

This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.1M Tris, 0.1M Tricine and 0.1% SDS pH 8.3-8.6.

Storage: 4 ° C

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MX-0948-01Tris-Tricine SDS Buffer 10X250 ml

This buffer is used as Agarose Gel Electrophoresis buffer. The buffer at 1X concentration contains 0.02MTris, 0.1M Borate and 1mM EDTA pH7.6

Storage: 4 ° C

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MX-0949-01Tris-Borate EDTA Buffer 5X200 ml

This buffer is used as electro-transfer buffer for protein blotting from SDS-PAGE. The buffer at 1x concentration contains 15mM Tris and 120mM Glycine, 0.1% SDS and 20% Methanol pH 8.6.

Storage: 4 ° C

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MX-0950-01Western Blotting Buffer 5X500ml

Sample buffer at 5x concentration contains 4% SDS, 20% Glycerol, 10% b -mercaptoethanol, 0.004% bromophenol blue and 0.125M Tris-HCl, pH approximately 6.8. This is used for preparing sample for SDS-PAGE

Storage: 4 ° C

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MX-0951-01Sample Buffer for SDS PAGE 5X5 x 12ml

Sample buffer at 5X concentration contains 20% Glycerol, 0.004% bromophenol blue and 0.125M Tris-HCl pH approximately 6.8. This is used for preparing sample for Native PAGE.

Storage: 4 ° C

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MX-0952-01Sample Buffer for Native PAGE 5X5 x 12 ml

Content of one bottle of brilliant blue G stain is to be made to a final volume of 1litre with deionized H 2 O. The final solution will contain 0.1% (w/v) brilliant blue G, 25% methanol and7%Acetic acid. This is used for staining protein in PAGE and Agarose gels.

Storage: 4 ° C

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MX-0953-01Brilliant Blue G Stain 2X500 ml

Content of one bottle of brilliant blue R stain is to be diluted to one litre with deionized H 2 O. The final solution contains 0.25% (w/v) brilliant blue R, 40% methanol and 7% Acetic acid. This is suitable for staining protein in polyacrylamide and agarose gels.

Storage: Room Temperature

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MX-0954-01Brilliant Blue R Stain 2X500 ml

After dilution to 1X concentration the solution will contain 0.1% (w/v) amido black, 25% (w/v) isopropanol and 10% Acetic acid. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE visualizes proteins in microgram range with a clear background.

Storage: Room Temperature

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MX-0955-01Amido Black Stain 2X250 ml

After dilution to 1X concentration the solution will contain 0.2% ponceau’s, 3%TCA. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE. Can visualize proteins in microgram range with a clear background.

Storage: Room Temperature

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MX-0956-01Ponceau’s Stain 2X250 ml

Content of one bottle of destainer is to be diluted 4 times with deionized water. The final solution contains 15% methanol and 7.5% Acetic acid. This is suitable for destaining polyacrylamide and agarose gels.

Storage: Room Temperature

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Cat.NoProductUnits
MX-0957-01Destainer 4X1 litre

Protein markers

MEDOX-PROProtein Markers consist of a novel set of proteins with defined sizes at convenient intervals for accurate protein size determination. The molecular weight of the markers ranges from 205,000 Da to 2300 Da, which is designed for routine and usual use in SDS-polyacrylamide gel electrophoresis. The MEDOX-PRO Protein Markers enable highly accurate size determination of unknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the MEDOX-PRO Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous “fuzzy” bands, or inaccurate size estimation.

The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).The MEDOX-PRO Protein Markers are available as solutions in which all the proteins are supplied at a concentration of 0.5 to 1mg/ml.

Storage: -20 ° C

Protein

Molecular Weight on
SDS-PAGE (Da)

Myosin

2,05,000

b-galactosidase

1,16,000

Bovine Serum Albumin

66,000

Ovalbumin

45,000

Carbonic anhydrase

29,000

Soyabean trypsin inhibitor

20,100

Lysozyme

14,400

Aprotinin

6,500

Insulin (a & b chains)

3,000

 

(2,300 – 3,400)

Ordering Information
Cat.NoProductUnits
MX-0211-01Protein Molecular weight Marker-Broad Range250 ul
MX-0211-02Protein Molecular weight Marker-Broad Range500 ul
MX-0212-01Protein Molecular weight Marker-High Range500 ul
MX-0213-01Protein Molecular weight Marker-Medium Range500 ul
MX-0214-01Protein Molecular weight Marker-Low Range500 ul
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Cat.NoProductUnits
MX-1077-01Native-PAGE Protein Marker (High Range)500 ul

The medium range markers are suitable to determinate molecular weight of SDS-PAGE separated polypeptides, stained by silver staining. Proteins supplied in this marker ranges from 6000 Da to 1,16,000 Da. The marker is formulated to yield a distribution of well-defined bands of approximately equal intensity with low background after electrophoresis and staining using Medox-Bio Silver Staining Kit.

Protein

Molecular Weight on
SDS-PAGE (Da)

b -galactosidase

1,16,000

Bovine Serum Albumin

66,000s

Ovalbumin

45,000

Carbonic anhydrase

29,000

Lysozyme

14,400

Aprotinin

6,500

Storage: -20 ° C

Ordering Information
Cat.NoProductUnits
MX-0251-01Protein Molecular Weight Marker, Medium range for Silver staining250 ul

Immunoelectrophoresis reagents

Tris Calcium Lactate buffer is used to run Rocket, Counter current and other immuno electrophoresis at the concentration of 1X.

Storage: RoomTemperature

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Cat.NoProductUnits
MX-0218-01Tris-Tricine Calcium Lactate Buffer 10X100 ml

Phosphate Buffer Saline is used in most of the immuno techniques at the concentration of 1X.

Storage: Room Temperature

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Cat.NoProductUnits
MX-0219-0110X PBS250ml

Specialized in Manufacturing & Supplying Products for Research.

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