100, 200, 300, 400, 500×3, 600, 700, 800, 900, 1000 x2 and 1500bp

The intensities of the bands of 500bp, 1000bp are increased to yield internal reference indicators.

Concentration : 200µg/ml

Storage Buffer :1XTE (10mMTris-HCl, pH8.0, 1.0mM EDTA)

6X Loading Buffer : 30% Glycerol,

Storage: -20°C

9 DNA fragments: 100, 200, 300, 400, 500, 600, 800,1000 and 1500bp.

Concentration : 540ng / 5µl

Recommended loading volume : 5µl

Storage Buffer : 1XTE (10mMTris-HCl, pH8.0, 1.0mM EDTA)

6X Loading Buffer :

Storage: -20°C

16 DNA fragments: 100, 200, 300, 400, 500, 600, 800, 1000, 1500, 2 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8kb, and 10 kb

DNA Logical-Ladder is consisted of 16 DNA fragments. The DNA Logic-Ladder was formulated to yield such a pattern that each band contains a Logic amount of the DNA fragment that correlates to its size. The Logic-ladder not only reveals a unique pattern for easy recognition of the bands on the gel, but also gives a quantitative gradient of the DNA amount. Thus, to measure both the size and the quantity of a particular DNA fragment could be achieved at a glance.

Recommended loading volume : 5µl

Storage Buffer : 1XTE (10mM Tris-HCl, pH8.0, 1mM EDTA)

6X Loading Buffer :

0.12% bromophenol blue,

Storage: -20°C

9 DNA fragments: 0.5kb, 1 kb, 2 kb, 3 kb, 4 kb, 5 kb, 6kb, 8kb and 10kb.

DNA 1Kb ladder is consisted of the 9 DNA fragments. The intensity of the 3kb band has been increased to yield an internal reference indicator.

Concentration : 250ng / 5µl

Recommended loading volume: 5µl

Storage Buffer : 1XTE (10mM Tris-Hcl, pH8.0, 1mM EDTA)

6x Loading Buffer :

30% Glycerol,

0.12% bromophenol blue,

0.12% xylene cyanol

Storage: -20°C

Six DNAFragments as 3530*,4878, 5643, 5804,7421 and 21,226*bp Supplied with 6X Loading dye solution

Lambda DNA (cI857,Sam7) was completely digested by EcoRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH7.6) and1mM EDTA.

EcoRI digest of Lambda DNA yields the following 6 DNA fragments

Concentration : 0.3-0.5mg/ml

6X Loading Dye Solution :

Storage: -20°C

*Note: The fragments marked by * may form additional bands, can be separated by heating 65°C for 5 min and then cooling on the ice for 3min.

7 DNA fragments: 23130*, 9416, 6557, 4361*, 2322, 2027 & 564bp

Lambda DNA was completely digested by HindIII, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH7.6) and 1mM EDTA. Hind III digest of Lambda DNA yields 7 DNA fragments

Concentration: 550ng / 6µl

Recommend loading volume: 6µl

Storage Buffer :

Storage: -20°C

*Note : The cohesive ends (12bp cos site of bacteriophage Lambda) of fragments 23130bp and 4361bp may anneal to and form the additional band. Fragments (marked by *) may be separated by heating to 65°C for 5 min and then cooling on ice for 3 min.

14DNA Fragments: 117 224, 702, 1264, 1371, 1929, 2323, 3675, 4324, 4822, 5687*, 6369, 7242, 8453*bp

0.05mg for 100 applications
Supplied with 6X Loading dye solution

Lambda DNA was completely digested by both EcoR 911, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

*Note: The fragments marker may form additional bands, can be separated by heating to 65°C for 5mins and then cooling on the ice for 3mins.

Concentration : 0.3-0.5mg/ml

Storage Buffer : 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

6X Loading Dye Solution:

0.25% bromophenol blue,

0.25% xylene cyanol,

30% glycerol and 50mM EDTA.

Storage: -20°C

12 DNA Fragments: 564, 831, 947, 1375, 1584, 1904, 2027, 3530*, 4268, 4973, 5148, 21226bp

0.05mg for 100 applications

Supplied with 6X Loading dye solution Lambda DNA was completely digested by both EcoRI and HindIII , phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

EcoRI & HindIII digest of Lambda DNA yields 12 DNA fragments

Storage Buffer: 10mM Tris-HCl (pH 8.0) and 1mM EDTA.

6X Loading Dye Solution:

0.25% xylene cyanol,

Storage: -20°C