Supplied with 10X Reaction Buffer(Ligation Buffer) T4 DNA ligase catalyzes a joining reaction between DNA fragment, involving the 3′-OH and the 5′-phosphate termini. The reaction requires ATP and Mg 2+ . The enzyme also catalyzes the exchange of free pyrophosphate into an activated carbon absorbable compound.
Concentration : 100U/µl
Unit Definition : 1 unit is defined as the amount of enzyme required to 50% ligation of Hind III-fragments of lambda DNA in 30 minutes at 16 ° C in 20µl of reactions mixture with DNA concentration about 300µg/ml
Reaction Buffer : 50mM Tris-HCl (pH 7.8), 10mM MgCl 2 , 10mM DTT, 1mM ATP and 25µg/ml BSA
Storage Buffer : 20mM Tris-HCl (pH 7.5), 50mM KCl, 10mM 2-Mercaptoethanol, 1mM EDTA and 50% glycerol
Storage : -20°C
| Ordering Information |
| Cat.No | Product | Units |
| MX-0702-01 | T4 DNA Ligase | 2000U |
| MX-0702-02 | T4 DNA Ligase | 4000U |
| MX-0702-03 | T4 DNA Ligase | 20000U |
