Principle

This kit is designed as a quick one step method for DNA extraction from bacteria. This isolated DNA act as DNA template for PCR amplification. This yield enough to 50 PCR reactions set up.

Features & Application

• The rapid isolation of DNA from both gram-positive and gram-negative organisms.
• The isolated DNA is suitable for medical diagnosis of PCR amplifications.
• No binding resin-avoids shearing
• No RNase treatment required
• Does not involve organic solvent extractions. (ex. Phenol, chloroform)

Kit Components & Storage

DNA Extraction solution Kit Protocol

Principle

This MX-10 spin column mini-preps kit is designed for a fast isolation of genomic DNA from clinical samples such as fresh or frozen whole blood (with common anti-coagulants such as citrate, EDTA, and heparin), serum, bone, other body fluids and cell suspensions. 200µl-400µl per column of healthy whole blood sample is enough to obtain 6-12µg of pure genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the MX-10 Column. Purified blood genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.

Applications

• Extraction of Genomic DNA from whole blood and other body fluids.

Features

• High quality of genomic DNA from blood and other clinical samples
• High yield and Reproducible
• No phenol / chloroform extraction , no ethanol precipitation

Storage

With the exception of the Proteinase K, the kit may be stored at room temperature. The proteinase K should be stored at -20ºC. The kit is stable for 12 months at room temperature. For longer storage, keep all contents cold.

Principle

This kit is used for rapid isolation and effectively reproducible, high yield og genomic DNA from blood sample

Features & Application

• The DNA extracted from 200 ul of blood sample.
• The isolated DNA is highly pure form, its suitable for PCR applications.
• No binding resin-avoids shearing
• No RNase treatment required
• Does not involve organic solvent extractions. (ex. Phenol, chloroform)

Kit Components & Storage

• Cell lysis buffer
• DNA Extraction solution
• Kit Protocol

Principle

This kit is designed for fast isolation of genomic DNA from animal tissues. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides, proteins, salts and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.

Applications

• Genomic DNA purification from different sources
• PCR
• Southern Blotting
• RAPD, AFLP or RFLP

Features

• Preparation of high quality genomic DNA from variable sources.
• Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
• High yield and Reproducible
• No phenol / chloroform extraction , no ethanol precipitation

Procedure

Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.

Gel electrophoresis of a animal genomic DNA sample purified by using EZ-10 Spin Column Genomic DNA Minipreps Kit. The lane at the left end is DNA marker.

Principle

This kit is designed for fast isolation of genomic DNA from plant tissues. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides,proteins, salts and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.

Applications

• Genomic DNA purification from different sources
• PCR
• Southern Blotting
• RAPD, AFLP or RFLP

Features

• Preparation of high quality genomic DNA from variable sources.
• Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
• High yield and Reproducible
• No phenol / chloroform extraction, no ethanol precipitation

Procedure

Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.

Gel electrophoresis of a plant genomic DNA sample purified by using MX-10 Spin Column Genomic DNA Minipreps Kit. The lane at the left end is DNA marker.

The Plant material is suspended in CTAB genomic DNA extraction buffer. Cells are then lysed by osmotic shock and salt precipitate the cell debris. Proteins are then precipitated using phenol chloroform and followed by ethanol precipitation of DNA.

Features & Application

• Mainly used to prepare pure high molecular weight DNA for gene manipulation.
• Used to construct genomic DNA libraries and to probe the plant genome for the presence of DNA markers such as RAPD and RFLP.

Principle

This kit is designed as a quick one step method for DNA extraction from bacteria. This isolated DNA act as DNA template for PCR amplification. This yield enough to 50 PCR reactions set up.

Features & Application

• Rapid isolation of DNA from monocot and dicot leaves.
• The highly pure isolated DNA is suitable for PCR application.
• No binding resin – avoids shearing
• No RNase treatment required
• Does not involve organic solvent extractions (ex. Phenol, chloroform)

Kit Components & Storage

• Cell lysis buffer
• DNA Extraction solution
• Kit Protocol

(For purification of PCR products and DNA cleanup of variable enzymatic reactions.)