PCR Reagents and Kits

Products

Taq Polymerase & Master Mix

 

SuperTherm DNA polymerase is a Thermostable DNA polymerase, purified fromThermus aquaticus strain by several rounds of chromatography. The purity of SuperTherm DNA polymerase is more than 90% of the total protein in the preparation. Amplification of DNA fragments (100bp to 5kb) can be achieved with it. The enzyme has both, 5′-3′ polymerase and 5′-3′ exonuclease activities.

Concentration : 5units /µl & 20units /µl

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0647-01SuperTherm Taq DNA Polymerase (5u/ul)100U
MX-0647-02SuperTherm Taq DNA Polymerase (5u/ul)250U
MX-0647-03SuperTherm Taq DNA Polymerase (5u/ul)1000U
MX-0647-04SuperTherm Taq DNA Polymerase (20u/ul)250U
MX-0647-05SuperTherm Taq DNA Polymerase (20u/ul)1000U
MX-0647-06SuperTherm Taq DNA Polymerase10 x 1000u

Pfu DNA Polymerase is a thermostable enzyme, possessing 5′-3′ DNA Polymerase and 3′-5′ proof-reading exonuclease activities. It is isolated from the hyperthermophylic marine archae Pyrococcus furiosis ( Pfu ). Pfu DNA Polymerase provides extremely high fidelity and can amplified larger DNA fragments (>20 Kb).

Concentration : 5units /µl

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0645-01SuperProof DNA Polymerase (Pfu) for Long PCR200U
MX-0645-02SuperProof DNA Polymerase (Pfu) for Long PCR1000U

HotStart DNA polymerase is a chemically modified form of the enzyme designed for Hot-Start PCR. The enzyme is highly purified and is free of nonspecific endo- or exo-nucleases.

Concentration : 5units /µl & 20units /µl

Storage : -20°C

Ordering Information
Cat.No Product Units
MX-0646-01 SuperHot DNA Polymerase (Hot Start) (5u/ul) 250U
MX-0646-02 SuperHot DNA Polymerase (Hot Start) (5u/ul) 1000U
Ordering Information
Cat.NoProductUnits
MX-1800-01Medox PCR Master Mix (2X)1ml (40rxns)
MX-1800-02Medox PCR Master Mix (2X)5 X 1ml (200rxns)
MX-1800-05Medox PCR Master Mix (2X) with red dye1ml (40rxns)
MX-1800-06Medox PCR Master Mix (2X) with red dye5 X 1ml (200rxns)
MX-1801-01Medox Pfu PCR Master Mix (2X)1ml (40rxns)
MX-1801-02Medox Pfu PCR Master Mix (2X)5 X 1ml (200rxns)
MX-1802-01Medox Hot Start PCR Master Mix (2X)1ml (40rxns)
MX-1802-02Medox Hot Start PCR Master Mix (2X)5 X 1ml (200rxns)
MX-1803-01Medox SYBR Green qPCR Master Mix (2X)1ml (40rxns)
MX-1803-02Medox SYBR Green qPCR Master Mix (2X)5 X 1ml (200rxns)
MX-1803-03Medox SYBR Green qPCR Master Mix (2X) with Low Rox1ml (40rxns)
MX-1803-04Medox SYBR Green qPCR Master Mix (2X) with Low Rox5 X 1ml (200rxns)
MX-1803-05Medox SYBR Green qPCR Master Mix (2X) with High Rox1ml (40rxns)
MX-1803-06Medox SYBR Green qPCR Master Mix (2X) with High Rox5 X 1ml (200rxns)

PCR Kits

Ordering Information
Cat.NoProductUnits
MX-0614-01DNA Amplification kit50 rxns
Ordering Information
Cat.NoProductUnits
MX-0615-01PCR Core kit100 rxns
MX-0615-02PCR Core kit50 rxns

PCR Reagents

DNA Amplification kit is used for the amplification of DNA fragments (100bp to 5kb). This kit contains all necessary components including Marker to perform PCR reactions. The reagents provided in this kit is sufficient for 50 PCR reactions except for control

DNA and Primer which are sufficient for 10 reactions.

Materials Provided
  • Taq DNA Polymerase
  • dNTPs
  • 10X Reaction Buffer
  • 25mM MgCl 2
  • Control DNA
  • Primer (Forward and Reverse) Match only to Control DNA
  • DNA Marker
  • Instruction Manual

Storage: -20 ° C

Ordering Information
Cat.NoProductUnits
MX-0614-01DNA Amplification kit50 rxns

PCR Core kit is used for the amplification of DNA fragments (100bp to 5kb). This kit contains all necessary components to perform PCR reactions. The reagents provided in this kit are sufficient for 100 PCR reactions.

Materials Provided
  • Taq DNA Polymerase
  • dNTPs
  • 10X Reaction Buffer
  • 25mM MgCl2
  • DNA Marker
  • Instruction Manual

Storage: -20 ° C

Ordering Information
Cat.NoProductUnits
MX-0615-01PCR Core kit100 rxns
MX-0615-02PCR Core Kit50 rxns

Purity by HPLC: >98.0%

PH: 6.8-7.0

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0828-01dNTP Mix Solution (10mM)100ul
MX-0828-02dNTP Mix Solution (10mM)2 00ul
MX-0828-03dNTP Mix Solution (10mM)1ml

Purity by HPLC: >98.0%

PH: 6.8-7.0

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0819-01dGTP Solution (100mM)1ml
MX-0819-02dGTP Solution (100mM)100μl
Ordering Information
Cat.NoProductUnits
MX-1066-01PCR Application Kit50rxns

Contains a set of dATP,dCTP & dTTP each 25mM

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0831-01dNTP set (10mM)4X100μl
MX-0831-02dNTP set (10mM)4X250μl
MX-0831-03dNTP set (10mM)4X500μl
MX-0831-04dNTP set (10mM)4X1 ml

Purity by HPLC: >98.0%

PH: 6.8-7.0

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0829-01d NTP Mix Solution (100mM)100ul
MX-0829-02dNTP Mix Solution (100mM)1ml

Contains a set of dATP, dGTP, dCTP & dTTP each 25µmol

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0830-01dNTP Set (100mM)4 X 100μl
MX-0830-02dNTP Set (100mM)4 x 250μl
MX-0830-03dNTP Set (100mM)4 x 50μl

Purity by HPLC: >98.0%

PH: 6.8-7.0

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0817-01dATP Solution (100mM)1ml
MX-0817-02dATP Solution (100mM)100 μl

Purity by HPLC: >98.0%

PH:7.0±0.5

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0818-01dCTP Solution (100mM)1ml
MX-0818-02dCTP Solution (100mM)100μl

Purity by HPLC: >98.0%

PH: 6.8-7.0

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0820-01dTTP Solution (100mM)1ml
MX-0820-02dTTP Solution (100mM)100μl

15mM MgCl2

PH:8.30

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0656-0110X PCR Buffer1ml

15mM and 25mM Mgcl for PCR optimization

Storage: -20°C

Ordering Information
Cat.NoProductUnits
MX-0648-01MgCl2 25mM5 X 1ml
MX-0648-02MgCl2 15mM5 X 1ml

RT-PCR

Supplied with 5X Reaction Buffer

Purified from an E.coli strain carrying a Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase overproducing plasmid. M-MuLV is an RNA and DNA dependent DNA polymerase. The enzyme also exhibits RNase H activity, catalyzes 5′ to 3′ synthesis of DNA. The enzyme exhibits 3′ to 5′ exonuclease activity.

Unit Definition : 1 unit of enzyme catalyzes the incorporation of 1 nanomoles of dTTP into acid insoluble form in 10 min at 37°C using poly(Ra) oligo(dT) as the template primer

Concentration : 50U/µl

Reaction Buffer : 50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT

Storage Buffer : 50mMTris-HCl (pH 8.3),1mM EDTA, 0.1mM DTT, 0.1% NP-40, 0.1mM NaCl and 50% glycerol

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0717-02M-MuLV Reverse Transcriptase1000U
MX-0717-03M-MuLV Reverse Transcriptase5000U
MX-0717-04M-MuLV Reverse Transcriptase10000U
Ordering Information
Cat.NoProductUnits
MX-1804-01Medox RT-PCR Kit (cDNA Synthesis Kit)10 rxns
MX-1804-02Medox RT-PCR Kit (cDNA Synthesis Kit)20rxns
Ordering Information
Cat.NoProductUnits
MX-1807-01Medox One Step RT-PCR Kit50rxns
MX-1807-02Medox One Step RT-PCR Kit100rxns
Ordering Information
Cat.NoProductUnits
MX-1805-01Oligo (dT) 15 Primer (50 μM)20 μl
Ordering Information
Cat.NoProductUnits
MX-1806-01Random Hexamer Primer 6-mer (50 μM)20 μl
Ordering Information
Cat.NoProductUnits
MX-1809-01RNA Stabilization solution100ml

Human Placental Source, Biotechnology Grade

Purity : >95%

DNase (exonuclease, endonuclease) : None detected

Concentration : 10units/ml

Storage buffer : 20mM HEPES-KOH, pH 7.6, 50mM KCl, 8mM DTT and 50% glycerol

Storage : -20°C

Ordering Information
Cat.No Product Units
MX-0632-01 RNase Inhibitor 500U
MX-0632-02 RNase Inhibitor 1000U

Primers/Oligonucleotides synthesis

SuperTherm DNA polymerase is a Thermostable DNA polymerase, purified fromThermus aquaticus strain by several rounds of chromatography. The purity of SuperTherm DNA polymerase is more than 90% of the total protein in the preparation. Amplification of DNA fragments (100bp to 5kb) can be achieved with it. The enzyme has both, 5′-3′ polymerase and 5′-3′ exonuclease activities.

Concentration : 5units /µl & 20units /µl

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0647-01SuperTherm Taq DNA Polymerase (5u/ul)100U
MX-0647-02SuperTherm Taq DNA Polymerase (5u/ul)250U
MX-0647-03SuperTherm Taq DNA Polymerase (5u/ul)1000U
MX-0647-04SuperTherm Taq DNA Polymerase (20u/ul)250U
MX-0647-05SuperTherm Taq DNA Polymerase (20u/ul)1000U

Pfu DNA Polymerase is a thermostable enzyme, possessing 5′-3′ DNA Polymerase and 3′-5′ proof-reading exonuclease activities. It is isolated from the hyperthermophylic marine archae Pyrococcus furiosis ( Pfu ). Pfu DNA Polymerase provides extremely high fidelity and can amplified larger DNA fragments (>20 Kb).

Concentration : 5units /µl

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0645-01SuperProof DNA Polymerase (Pfu)200U
MX-0645-02SuperProof DNA Polymerase (Pfu)1000U

HotStart DNA polymerase is a chemically modified form of the enzyme designed for Hot-Start PCR. The enzyme is highly purified and is free of nonspecific endo- or exo-nucleases.

Concentration : 5units /µl & 20units /µl

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0646-01SuperHot DNA Polymerase (Hot Start) (5u/ul)250U
MX-0646-02SuperHot DNA Polymerase (Hot Start) (5u/ul)1000U
MX-0646-03SuperHot DNA Polymerase (Hot Start) (20u/ul)250U
MX-0646-04SuperHot DNA Polymerase (Hot Start) (20u/ul)1000U
Ordering Information
Cat.NoProductUnits
MX-0639-015X Medox PCR Master Mix (1.5mM MgCl 2 )100 x50µlrxns
MX-0639-025X Medox PCR Master Mix (1.5mM MgCl 2 )1000 x50µlrxns
MX-0643-015X Hot-Start Medox PCR Master Mix with 1.5mM MgCl 2100 x50µlrxns
MX-0643-025X Hot-Start Medox PCR Master Mix with 1.5mM MgCl 21000 x50µlrxns
Ordering Information
Cat.NoScalePurification MethodODPurity
MX-100115 nmolColumm5.0098%
MX-1002200 nmolColumm10.0098%
MX-10031 μmolColumm30.0098%
MX-100450 nmolPAGE2.0099%
MX-1005200 nmolPAGE8.0099%
MX-10061 μmolPAGE25.0099%
MX-100750 nmolDHPLC2.0099%
MX-1008200 nmolDHPLC8.0099%
MX-10091 μmolDHPLC25.0099%

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