PCR Reagents and Kits

Products

Taq Polymerase & Master Mix

 

SuperTherm DNA polymerase is a Thermostable DNA polymerase, purified fromThermus aquaticus strain by several rounds of chromatography. The purity of SuperTherm DNA polymerase is more than 90% of the total protein in the preparation. Amplification of DNA fragments (100bp to 5kb) can be achieved with it. The enzyme has both, 5′-3′ polymerase and 5′-3′ exonuclease activities.

Concentration : 5units /µl & 20units /µl

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0647-01SuperTherm Taq DNA Polymerase (5u/ul)100U
MX-0647-02SuperTherm Taq DNA Polymerase (5u/ul)250U
MX-0647-03SuperTherm Taq DNA Polymerase (5u/ul)1000U
MX-0647-04SuperTherm Taq DNA Polymerase (20u/ul)250U
MX-0647-05SuperTherm Taq DNA Polymerase (20u/ul)1000U
MX-0647-06SuperTherm Taq DNA Polymerase10 x 1000u

PCR Kits

Ordering Information
Cat.NoProductUnits
MX-0614-01DNA Amplification kit50 rxns

PCR Reagents

DNA Amplification kit is used for the amplification of DNA fragments (100bp to 5kb). This kit contains all necessary components including Marker to perform PCR reactions. The reagents provided in this kit is sufficient for 50 PCR reactions except for control

DNA and Primer which are sufficient for 10 reactions.

Materials Provided
  • Taq DNA Polymerase
  • dNTPs
  • 10X Reaction Buffer
  • 25mM MgCl 2
  • Control DNA
  • Primer (Forward and Reverse) Match only to Control DNA
  • DNA Marker
  • Instruction Manual

Storage: -20 ° C

Ordering Information
Cat.NoProductUnits
MX-0614-01DNA Amplification kit50 rxns

RT-PCR

Supplied with 5X Reaction Buffer

Purified from an E.coli strain carrying a Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase overproducing plasmid. M-MuLV is an RNA and DNA dependent DNA polymerase. The enzyme also exhibits RNase H activity, catalyzes 5′ to 3′ synthesis of DNA. The enzyme exhibits 3′ to 5′ exonuclease activity.

Unit Definition : 1 unit of enzyme catalyzes the incorporation of 1 nanomoles of dTTP into acid insoluble form in 10 min at 37°C using poly(Ra) oligo(dT) as the template primer

Concentration : 50U/µl

Reaction Buffer : 50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT

Storage Buffer : 50mMTris-HCl (pH 8.3),1mM EDTA, 0.1mM DTT, 0.1% NP-40, 0.1mM NaCl and 50% glycerol

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0717-02M-MuLV Reverse Transcriptase1000U
MX-0717-03M-MuLV Reverse Transcriptase5000U
MX-0717-04M-MuLV Reverse Transcriptase10000U

Primers/Oligonucleotides synthesis

SuperTherm DNA polymerase is a Thermostable DNA polymerase, purified fromThermus aquaticus strain by several rounds of chromatography. The purity of SuperTherm DNA polymerase is more than 90% of the total protein in the preparation. Amplification of DNA fragments (100bp to 5kb) can be achieved with it. The enzyme has both, 5′-3′ polymerase and 5′-3′ exonuclease activities.

Concentration : 5units /µl & 20units /µl

Storage : -20°C

Ordering Information
Cat.NoProductUnits
MX-0647-01SuperTherm Taq DNA Polymerase (5u/ul)100U
MX-0647-02SuperTherm Taq DNA Polymerase (5u/ul)250U
MX-0647-03SuperTherm Taq DNA Polymerase (5u/ul)1000U
MX-0647-04SuperTherm Taq DNA Polymerase (20u/ul)250U
MX-0647-05SuperTherm Taq DNA Polymerase (20u/ul)1000U

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