Description

Bacterial protein extraction kit is designed for the lysis of bacterial cells for the purification of recombinant and wild type proteins. It is useful for the preparation of high purity inclusion bodies. There is no need for special equipment such as sonicator. This method is fast and obtains high yields of protein. The gentle, non-ionic extraction method can be used to extract functionally soluble proteins without a need for secondary treatment such as sonication or freeze/thaw. It is compatible with the FLAG, poly-His and GST fusion protein affnity chromatography purification systems, the kit is also compatible with many enzyme or protein assays and compatible with protease inhibitor cocktails.

Applications

The kit provides reagent for extracting bacterial protein from 1-5litre medium

Features

• Simple & Fast, no need for special equipment
• SIt is suitable for long-term storage

Storage Condition

• Store 10X Cell lysis buffer at 4 ° C;
• DNase I, RNase, Lysozyme and PMSF should bestored at -20 ° C.

Description

Extraction of cell proteins requires efficient cell lysis and protein solubilization, while avoiding protein degradation and interference with the proteins’ immuno reactivity and biological activity. The Mammalian protein extraction Kit is composed of solutions to be mixed and used for lysis of adherent cells, nonadherent cells and tissues. It enables efficient solubilization of proteins for analysis and a low background in immuno precipitation assays. The kit has been tested on many types of cells and tissues.

Applications

The Mammalian Protein Extraction kit provides reagents to extract proteins of 5 x (10 7 -10 8 ) mammalian cells or 1g tissues, which contains buffer, detergents, NaCl and protease inhibitor cocktail solutions. This enables the researcher to change the buffer’s components to obtain maximum efficiency for a specific protein

Features

• Convenient to change the component of the detergents in the extraction buffer
• Efficient solubilization of proteins with low background in immunoprecipitation

Storage Condition