MEDOX-BIO Submarine (Horizontal) Gel Systems

Nucleic acids are routinely separated according to their mass and structural properties by electrophoresis on Agarose gels. Linear double stranded DNA has a constant negative charge per unit length of molecule, resulting from the phosphate backbone of the helix. Thus, they migrate towards the anode during electrophoresis at a rate that is inversely proportional to the log of the molecular mass. Electrophoretic separation of nucleic acids therefore is strictly according to size. The molecular mass of double stranded DNA fragments can be determined after electrophoresis by comparison with standards of known molecular masses.

Submarine gels, often called Horizontal agarose gels, are routinely used in the laboratory to analyze DNA. Agarose gels are submerged in electrophoresis buffer in a horizontal electrophoresis apparatus. The buffer and gel form a current bridge between the two electrode chambers. Ethidium Bromide is mixed in the gel matrix to enable fluorescent visualization of the DNA fragments under UV light after electrophoresis.

In addition to its importance as an analytical tool, gel electrophoresis is widely used for isolating and then purifying specific fragments of DNA, usually in preparation for subcloning. Hence, this is a very commonly required procedure.

MEDOX-BIO Submarine Gel Systems are designed to suit various applications related to Nucleic acid separation in laboratories. These computer-designed systems are made out of well-polished and clear Plexiglass sheets fused together by using high-strength solvent adhesives. Our strict Quality control measures ensure the unit is leak-proof and withstand heat generated due to high voltage and longer running time.

Every Gel System has clear markings to indicate Buffer level in Gel tank.

The system is complete and supplied with all accessories required to run the gel.